Clinical and Basic Study on Human Erythrocyte Carbonic Anhydrase I-Presence of a Mixed Disulfide with Glutathione.

人红细胞碳酸酐酶 I-混合二硫化物与谷胱甘肽存在的临床和基础研究。

• 批准号: 60570547
• 负责人: KONDO Takahito
• 金额: $ 1.15万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570547/
• 关键词: Erythrocyte; Carbonic anhydrase I; Primary aldosteronism; グルタチオン

A low activity form of carbonic anhydrase I was found in erythrocytes of five patients with primary aldosteronism. The specific activity was very low, however the activity was restored by drug treatment as well as adrenalectomy.The enzyme was purified to homogeneity from one of the patients. The enzyme was not distinguishable with respect to its antigenecity and zinc content. On theother hand, the inhibition constant and binding affinity to a specific inhibito, acetazolamide, was quite different. Sulfhydryl titration, amino acid analysis and peptide mapping analysis suggested that the low activity from contains a mixed disulfide with glutathione which is closely associated with its low activity form. This result supports our previous findings on <gamma> -glutamyl cyclotransferase and N-acetyl hexosaminidase, both of which were found to contain a mixed disulfide with glutathione. The presence of a mixed disulfide in the protein seems to be a common phenomenon.

在5例原发性醛固酮增多症患者的红细胞中发现了一种低活性的碳酸酐酶I。比活性很低，但通过药物治疗和肾上腺切除术恢复了活性。该酶的抗原性和锌含量是不可区分的。另一方面，对乙酰唑胺的抑制常数和结合亲和力则有很大差异。巯基滴定、氨基酸分析和肽图谱分析表明，低活性形式含有与谷胱甘肽密切相关的混合二硫键。这一结果支持了我们之前关于<gamma>-谷氨酰环转移酶和N-乙酰己糖胺酶的发现，这两种酶都被发现含有与谷胱甘肽混合的二硫键。蛋白质中混合二硫化物的存在似乎是一种常见现象。

项目成果

近藤宇史: 含硫アミノ酸. 17. 241-248 (1984)

Ushi Kondo：含硫氨基酸 17. 241-248 (1984)。

Kondo, T.: "A novel low activity form of carbonic anhydrase I in erythrocytes of patients with primary aldosteronism." J. Biol. Chem.259. 15517-15522 (1984)

Kondo, T.：“原发性醛固酮增多症患者红细胞中碳酸酐酶 I 的一种新型低活性形式。”

Kondo,T.: J.Biol.Chem.259. 15517-15522 (1984)

近藤，T.：J.Biol.Chem.259。

Kondo, T.: "Erythrocyte carbonic anhydrase isozyme I which formed a mixed disulfide with glutathione." Sulfur Amini Acids. 17. 241-248 (1984)

Kondo, T.：“红细胞碳酸酐酶同工酶 I 与谷胱甘肽形成混合二硫键。”

Kondo,T.: Annals N.Y.Acad.Sci.429. 302-315 (1984)

Kondo,T.：《纽约学术年鉴》429。