Experimental Research of Canine Partial Liver Transplantation

我们的方法是利用来自活体和相关供体的肝移植用于犬部分肝移植。结果总结如下：1)采用体内灌注法研究移植体存活所需的体积。13例患者中有6例存活3个月以上，左外侧叶、左中央叶和尾状叶突灌注安全。由此可见，在全肝切除犬中，这3个肝叶被原位移植，经环孢素和甲基强的松龙治疗的8例中，有5例存活超过7天，最长存活时间为40天。为了使用活体供体，左中央叶和左外侧叶在体内灌注并原位移植。15例供体中有8例存活3周以上，供体手术安全完成。15例受者中有6例存活超过7天，目前最长存活时间为10天。因此，认为活体肝部分灌注的方法在临床上是适用的，并进一步提出，在供、受体体重接近相等的情况下，受体仅靠左外侧叶和左中央叶存活。

Our method was designed to utilize liver graft obtained from a living and related donor in canine partial liver transplantation.The results obtained are summarized as follows:1) The volume of graft necessary for survival was investigated by in vivo perfusion method. left lateral, left central lobe and processus of caudate lobe were perfused safely and 6 out of 13 cases survived for more than 3 months. From this result, these 3 lobes from sacrificed donor were orthotopically transplanted in total hepatectomised dogs, and 5 out of 8 cased treated by ciclosporin and methylpredonisolone survived for more than 7 days and the longest survival time was 40 days.2). In order to use a living donor, left central and left lateral lobe were perfused in vivo and transplanted orthotopically. Eight out of 15 donors survived for more than 3 weeks and the operation for donor was proved to be done safely.Six out of 15 recipients survived for more than 7 days and the longest survival time was 10 days at the present time.Thus, the mothod of in vivo partial liver perfusion was thought to be applicable clinically, and it was further suggested that the recipient could survive with only left lateral and left central lobe, when the weight of donor and recipient were nearly equal.