Experimental Research of Canine Partial Liver Transplantation

将活体及亲属供体的肝移植用于犬部分肝移植，结果如下：1)采用活体灌注法测定存活所需的移植物体积。左外侧、左中央叶及尾状叶突均安全灌注，6例存活3个月以上。因此，将供体的3个叶移植于全肝切除犬体内，经环孢素和甲基强的松龙治疗的8只犬中有5只存活超过7天，最长存活40天。为了使用活体供体，将左中叶和左外侧叶进行活体灌流，并进行原位移植。15例供体中8例存活3周以上，供体手术安全，6例供体存活7天以上，目前存活时间最长可达10天。因此，活体部分肝脏灌注法在临床上是可行的，并进一步提示在供体和受体体重相近的情况下，受体只需左侧和左侧中央叶即可存活。

Our method was designed to utilize liver graft obtained from a living and related donor in canine partial liver transplantation.The results obtained are summarized as follows:1) The volume of graft necessary for survival was investigated by in vivo perfusion method. left lateral, left central lobe and processus of caudate lobe were perfused safely and 6 out of 13 cases survived for more than 3 months. From this result, these 3 lobes from sacrificed donor were orthotopically transplanted in total hepatectomised dogs, and 5 out of 8 cased treated by ciclosporin and methylpredonisolone survived for more than 7 days and the longest survival time was 40 days.2). In order to use a living donor, left central and left lateral lobe were perfused in vivo and transplanted orthotopically. Eight out of 15 donors survived for more than 3 weeks and the operation for donor was proved to be done safely.Six out of 15 recipients survived for more than 7 days and the longest survival time was 10 days at the present time.Thus, the mothod of in vivo partial liver perfusion was thought to be applicable clinically, and it was further suggested that the recipient could survive with only left lateral and left central lobe, when the weight of donor and recipient were nearly equal.