Experimental Research of Partial Liver Transplantation

部分肝移植的实验研究

• 批准号: 01570701
• 负责人: SASAKI Mutsuo
• 金额: $ 1.41万
• 依托单位: Department of Surgery, Hirosaki University School of medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570701/
• 关键词: Canine partial liver transplantation; Graft volume; Perfusion injury; Experimental liver cirrhosis; 犬生体部分肝移植; シクロスポリン; 免疫抑制; 肝毒性; 胆管上皮細胞抗原; 豚生体部分肝移植; 免疫制御

Our method was designed to investigate the minimal graft volume in partial liver transplantation for adult recipients.The results obtained are summarized as follows :1)The minimal volume of graft necessary for survival was investigated by in vivo perfusion method. Left lateral and left central lobes(45% graft)were perfused safely and 10 out of il cases survived for more than 3 weeks. Thirteen cases out of 27 recipients with 45% graft survived for more than 7 days. Five cases died within 2 days and 9 cases died during the period between 2 to 7 days.2)In order to minimize the graft volume, left lateral lobe and caudate lobe(30% graft volume)were perfused in vivo. Although 9 out of 15 cases survived for more than 3 weeks, 4 cases died of hepatic failure within 3 weeks. Five recipients with 30% graft died of hepatic failure within 7 days. Thus 30% graft volume was thought to be the critical volume.3)Experimental liver cirrhosis was made by 99% ethanol fixation of the epiterial cells of the canine bile duct. Histological findings demonstrated the similarity of human alcholic liver cirrliosis. This method is thought to be simple and applicable to partial liver transplantation.

本实验采用活体灌注法研究了成人部分肝移植的最小肝移植体积，主要结果如下：1）采用活体灌注法研究了存活所需的最小肝移植体积。左外叶和左中叶（45%）安全灌注，10例存活3周以上。27例受者中13例存活7天以上，移植物成活率为45%。5例于术后2 d内死亡，9例于术后2 ~ 7 d死亡。2）为减少移植肝体积，采用左外叶和尾状叶（占移植肝体积的30%）进行体内灌注。15例中9例存活3周以上，4例于3周内死于肝功能衰竭。5例移植肝占30%的受者在7天内死于肝功能衰竭。因此，30%的移植体积被认为是临界体积。3）实验性肝硬化是通过99%乙醇固定狗胆管上皮细胞。组织学表现与人类酒精性肝硬化相似。本方法简便易行，适用于部分肝移植。

项目成果

佐々木 睦男,袴田 健一,千葉 茂夫,今 充: "イヌ部分肝移植モデルにおける肝容量および潅流障害について" 移植.

Mutsuo Sasaki、Kenichi Hakamada、Shigeo Chiba、Mitsuru Kon：“关于犬部分肝移植模型中的肝脏体积和灌注损伤”移植。

Mutsuo Sauaki: "Canine partial liver transplantation from living donor" Transplant Proc.

Mutsuo Sauaki：“来自活体捐赠者的犬部分肝移植”移植程序。

佐々木睦男: "リビングドナ-を用いた犬部分肝移植の実験的研究" 日本消化器外科学会雑誌. 21. 1203-1206 (1988)

Mutsuo Sasaki：“使用活体供体的犬部分肝移植的实验研究”日本胃肠外科学会杂志 21. 1203-1206 (1988)。

Mutsuo Sasaki, Ken Miyagishima, Shigeo Chiba, mitsuru Konn and Keiichi Ono: "Experimental Research of Split Liver Transplantation" Pediatric Surgery. 22. 974-978 (1990)

Mutsuo Sasaki、Ken Miyagishima、Shigeo Chiba、mitsuru Konn 和 Keiichi Ono：“裂体肝移植的实验研究”小儿外科。

Mutsuo Sasaki, Ken-ichi Hakamada, Shigeo Chiba and Mitsuru Konn: "Perfusion Injury in Experimental Canine Partial Liver Transplantation" Japanese Journal of Transplantation.

Mutsuo Sasaki、Ken-ichi Hakamada、Shigeo Chiba 和 Mitsuru Konn：“实验性犬部分肝移植中的灌注损伤”日本移植杂志。