Development of an artificial pancreas with hybridoma.

本项目主要完成了四个方面的工作：第一，建立了一种新的不经胶原酶消化的胰岛分离方法，命名为“冷冻分离法”;第二，建立了一种新的胰岛细胞培养细胞系<VX_2>-A Asahikawa/，并在该细胞系中建立了胰岛细胞杂交瘤的预定伴侣。第三，体外人工胰腺的建立。最后，建立了一种新的胰岛细胞和培养细胞的冷冻保存系统。该系统是一种使用液氦的超快速冷却方法，可在任何实验室中使用，成本最低。

Four Progects were done by this grant:First of all, A new isolation method of the islets from the pancreas without collagenase ddigestion was developed and named "Freeze Dissuptive Separation Method".Second, A new cultured cell line, <VX_2> -A Asahikawa/, in which a planned partner of an islet cell hybridoma was established. But, insulin-releasable hybridoma was not established.Third, An extra-corporal artificial pancreas was examined.Finally, A new cryopreservation system for isolated pancreas islets and cultured cells was developed. This system is an ultra-rapid cooling method using liquid helium that is available in any laboratory and of minimal cost.