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Development of the new combination therapy used by Super selective extra coporal local hyperthermia.

开发超选择性体外局部热疗所使用的新型联合疗法。

基本信息

批准号：
61870053
负责人：
HAYASHI Hirokazu
金额：
$ 6.34万
依托单位：
Asahikawa Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61870053/
关键词：
Extra-corporal local hyperthermia Induction hearing Microwave Magnetic fluid Zein TAE VX2-A細胞熱電対 V【X_2】腫瘍

项目摘要

The transcatheter arterial embolization (TAE) has been widely used as the conservative therapy for non-resectable liver tumor. However, due to the revascuralization to the tumor caused by the imcomplete TAE and the developing collateral arteries, its efficacy has been still unsatisfied. The new therapeutic method for non-operative liver tumoe was designed to apply extra-corporal local hyperthermia by the microwave of RF irradiation after TAE by Zein contained with metal particles or magnetic fluid which enhance the localized hyperthermic errect.This experimental study was undertaken to develope the appropriate embolic agents for this purpose and to assess the synergic cytotoxic effect to this combination therapy.Zein which belongs to prolamin and cobalt magnetic fluid were chosed as the permanent embolic substances. Metal particles which made from Tital of Stainless material were used as hyperthermic mediating agent. The radiation of 2450 MHZ microwave was employed for the extra-corpor … More al local hyperthermia. This work was done to examine as followd; 1) the quality of Zein for the aims of permanent TAE agent and metal particle coating substance, 2) the possibility to bring local hyperthermic condition on the metal particle embolized region, 3) the cytotoxic effect of this procedure using tumor-bearing Japanese white rabbits.Zein is dissolved in alkaline solution and coagulate smoothly by the lowering temperature, but that is irreversible even in the rewaming condition. Furthermor, no inflammatory reaction could be detected. But, the magnetic fluid caused inflammatory reactions. It is easy to detect the condition of TAE due to the radiopague metal particles and magnetic fuluid which also provide the advantage to follow-up the efficacy of the hyperthermic treatment. The remarkable temperature gradient was observed after the mictowave radiation between the part with and without metal particles in gelatin phantom or in liver tissue. The tendency of the increase of necrotized tumor involved region was detected in the experimental study of tumor bearing animals after this procedure.Cytotoxic effect by the hyperthermia is based on the functinal difference between normal vessels and tumor vessels and the differnce of sensibivity between normal cells and tumor cells of which the heat errect. Therefor, out new procedure which employs permanent TAE combined with hyperthermia in metal particles contained tumor involved region, might have the intense cytotoxic effect which proved by this region, might have th intense cytotoxic effect which proved by this experiment. Less

经导管肝动脉栓塞术(TAE)已被广泛应用于不能切除的肝肿瘤的保守治疗。但由于TAE不全及侧支循环发育对肿瘤的再通畅，其疗效仍不理想。非手术治疗肝肿瘤的新方法是在含有金属颗粒或磁性液体的玉米醇溶蛋白TAE后，用微波进行体外局部热疗，以增强局部热效应。本实验旨在研制合适的栓塞剂，并评价其协同细胞毒作用。以不锈钢为原料制得的金属颗粒作为加热介质。体外…采用2450兆赫兹微波辐射更多的局部体温升高。1)用于永久性TAE制剂和金属颗粒包衣材料的玉米醇溶蛋白的性质，2)金属颗粒栓塞区局部加热的可能性，3)荷瘤日本大白兔的细胞毒作用。玉米醇溶蛋白在碱性溶液中溶解，在较低的温度下可以顺利凝结，但即使在重温条件下这也是不可逆的。此外，未检测到炎症反应。但是，磁性液体引起了炎症反应。由于放射性粒子、金属微粒和磁流体的存在，TAE的病情易于检测，这也为热疗的疗效随访提供了有利条件。微波辐射后，明胶体模或肝组织中有金属颗粒的部分与无金属颗粒的部分之间有明显的温度梯度。在荷瘤动物的实验研究中发现，热疗后肿瘤坏死区有增加的趋势。热疗的细胞毒作用是基于正常血管和肿瘤血管之间的功能差异以及正常细胞和肿瘤细胞对热的敏感性的差异。因此，采用永久性TAE联合热疗的新方法，可能具有该区域所证明的强烈的细胞毒作用，也可能具有本实验所证明的强烈的细胞毒作用。较少