Role of Alveolar Type <II> Epithelial Cells in the Repairing Process after Lung Injury

肺泡<II>型上皮细胞在肺损伤后修复过程中的作用

• 批准号: 60570723
• 负责人: SUGAHARA Kazuhiro
• 金额: $ 1.34万
• 依托单位: KUMAMOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570723/
• 关键词: Alveolar type <II> epithelial cells; Surface active material; Lung injury; Repair; Growth factor; 人体材料

1. Cell isolation and culture: Alveolar type <II> cells were isolated from adult rat lungs by tissue dissociation with elastase and density gradient. The yield of the cells is about 20x <10^6> cells/rat and the purity of the adherent cells is 85-95 % type <II> cells. The cells appear similar lamellar bodies and organella to those in intact lung, by tannic acid stain and electron microscope. At the cytoskeleton structure examining by avidin-biotin stain, they have fine intermediate filaments such as keratin and actin. Those structure was affected by different substrates, especially fibronectin.2. Function of the cells: Alveolar type <II> cells form domes after confluent, which suggests that they have active ion transport. Alveolar type <II> monolayers on filter have bioelectronic properties of potential different(PD);-1.0 mV, resistance(R); 200 ohm・ <cm^2> ; short circuit current(SCC); 1.8 uA/ <cm^2> . The monolayers on fibronectin-coating filter have higher PD and R than those on the f … More ilter. Taken together, fibronectin is an important functional component of the extracellular matrix that supports alveolar type <II> cells during alveolar reepithelization.3. Surface active material secretion: To clarify the mechanism of surfactant secretion, we examined the effects of <beta> -stimulant and <Ca^(++)> on surfactant secretion.4. An animal model of lung injury: Exposure to 85 % oxygen rats had the proliferation and hypertrophy of alveolar type <II> cells. We have tried to find a factor which induces cell proliferation.5. Isolation of human alveolar type <II> cells from resected human lung.6. In vivo culture: Although the cells are necessarily damaged and lose surface receptors to some extent by the isolation procedure and conditions in vitro, we are trying to develop a new system of in vivo culture, which isolated cells are again implanted into the peritoneum or subcutaneous region of the different rat.7. Function of tracheal epithelium: The tracheal epithelial cells have some similar functions to those of alveolar type <II> epithelial cells, such as ion transport , mucus secretion and proliferation. Less

1.细胞分离和培养：<II>通过用弹性蛋白酶和密度梯度的组织解离从成年大鼠肺分离肺泡型细胞。细胞的产量约为20 × 10^6个细胞/大鼠，贴壁细胞的纯度为85- 95%型<II>细胞。鞣酸染色及电镜观察可见与完整肺组织相似的板层小体及细胞器。用亲和素-生物素染色法观察细胞骨架结构，可见角蛋白和肌动蛋白等细小的中间纤维。这些结构受不同底物的影响，尤其是纤维连接蛋白.细胞功能：肺泡型<II>细胞汇合后形成穹隆，具有主动的离子转运功能。滤膜<II>上的肺泡型单细胞膜具有电位差（PD）;-1.0 mV，电阻（R）; 200 ohm·cm 2;短路电流（SCC）; 1.8 uA/cm 2的生物电子学特性。纤维连接蛋白膜上的单层膜比纤维连接蛋白膜上的单层膜具有更高的PD和R ...更多信息 过滤器总之，纤连蛋白是细胞外基质的重要功能成分，<II>在肺泡上皮再生过程中支持肺泡型细胞.表面活性物质分泌：为了阐明表面活性物质分泌的机制，我们研究<beta>了刺激剂和&lt;Ca^（++）&gt;对表面活性物质分泌的影响。4.肺损伤动物模型：85%氧暴露大鼠肺泡型细胞增生肥大<II>。我们试图找到一种诱导细胞增殖的因子.从<II>切除的人肺中分离人肺泡型细胞.体内培养：虽然细胞在一定程度上受到体外分离程序和条件的损伤并失去表面受体，但我们正在尝试开发一种新的体内培养系统，将分离的细胞再次植入不同大鼠的腹膜或皮下区域。气管上皮的功能：气管上皮细胞具有与肺泡型上皮细胞相似的功能<II>，如离子转运、粘液分泌和增殖。少

项目成果

須加原一博: 環境科学研究広報. 37. 232-234 (1987)

Kazuhiro Sukahara：环境科学研究公共关系 37. 232-234 (1987)

須加原一博: 日本胸部疾患学会誌. 24. 110 (1986)

Kazuhiro Sukahara：日本胸部疾病学会杂志，24. 110 (1986)。

須加原一博: 呼吸. 6. (1987)

须贺原一宏：呼吸 6. (1987)

Kazuhiro SUGAHARA: "Ion transport by tracheal epithelium" Respiration Research(Kokyu). 6(6). (1987)

Kazuhiro SUGAHARA：“气管上皮的离子传输”呼吸研究（Kokyu）。