Role of Alveolar Type <II> Epithelial Cells in the Repairing Process after Lung Injury

Cell isolation and culture：Alveolar type <II> cells were isolated from adult rat lungs by tissue dissociation with elastase anddensity gradient. The yield of The cells is about 20x <10^6> cells/rat and The purity of Theadherent cells is 85-95 % type <II> cells. The cells appear similar lamellar bodies and organella tothose in intact lung，tannic acid stain and electron microscope At the cytoskeleton structure examining by tannic acid stain and electron microscopeavidin - biotin stain,他们有精细的中间filaments such as keratin and actin.那些结构是经过的different substrates, especially fibronectin.2. Function of the cells：Alveolar type <II> cells form domes after confluent，其中suggests that they have active ion transport. Alveolar type <II> monolayers on filter havepotential different生物电子特性（PD）；-1.0 mV, resistance(R)；200 ohm <cm^2>；short circuit current(SCC)；1.8 uA/ <cm^2> . The monolayers on fibronectin-coating filter have higher PD and R than those on Thef……More ilter. Taken together，fibronectin is an important functional component of the extracellular matrix that supports alveolartype <II> cells during alveolar reepithelization.3. Surface active material secretion：To clarify the mechanism of surfactant secretion我们examined the effects of <beta> -stimulant and <Ca^(++)> on surfactant secretion.4. An animalmodel of lung injury：85 % oxygen rats had the proliferation and hypertrophy of alveolar type <II> cells. We5. Isolation of human alveolar type发现一个因素which induces cell proliferationcells from resected human lung.6. In vivo culture：虽然the cells are necessarily damaged and lose surface receptors to some extent by theisolation procedure and conditions in vitro，we are trying to develop a new system of in体内文化，isolated cells are again implanted into the peritoneum or subcutaneous region of the differentrat.7. tracheal epithelium：The tracheal epithelial cells have some similar functions to those of alveolar type <II> epithelialcells, such as ion transport, mucus secretion and proliferation. Less

1. Cell isolation and culture: Alveolar type <II> cells were isolated from adult rat lungs by tissue dissociation with elastase and density gradient. The yield of the cells is about 20x <10^6> cells/rat and the purity of the adherent cells is 85-95 % type <II> cells. The cells appear similar lamellar bodies and organella to those in intact lung, by tannic acid stain and electron microscope. At the cytoskeleton structure examining by avidin-biotin stain, they have fine intermediate filaments such as keratin and actin. Those structure was affected by different substrates, especially fibronectin.2. Function of the cells: Alveolar type <II> cells form domes after confluent, which suggests that they have active ion transport. Alveolar type <II> monolayers on filter have bioelectronic properties of potential different(PD);-1.0 mV, resistance(R); 200 ohm・ <cm^2> ; short circuit current(SCC); 1.8 uA/ <cm^2> . The monolayers on fibronectin-coating filter have higher PD and R than those on the f … More ilter. Taken together, fibronectin is an important functional component of the extracellular matrix that supports alveolar type <II> cells during alveolar reepithelization.3. Surface active material secretion: To clarify the mechanism of surfactant secretion, we examined the effects of <beta> -stimulant and <Ca^(++)> on surfactant secretion.4. An animal model of lung injury: Exposure to 85 % oxygen rats had the proliferation and hypertrophy of alveolar type <II> cells. We have tried to find a factor which induces cell proliferation.5. Isolation of human alveolar type <II> cells from resected human lung.6. In vivo culture: Although the cells are necessarily damaged and lose surface receptors to some extent by the isolation procedure and conditions in vitro, we are trying to develop a new system of in vivo culture, which isolated cells are again implanted into the peritoneum or subcutaneous region of the different rat.7. Function of tracheal epithelium: The tracheal epithelial cells have some similar functions to those of alveolar type <II> epithelial cells, such as ion transport , mucus secretion and proliferation. Less