Role of Alveolar Type <II> Epithelial Cells in the Repairing Process after Lung Injury

1.细胞隔离和培养： Alveolar型<II>细胞通过组织隔离与弹性酶和密度梯度的成人大鼠午餐进行隔离。细胞的yield约为20 x &lt;10^6&gt;细胞/大鼠，而修饰细胞的纯度为85- 95%<II>。这些细胞看起来类似于晶状体的膜和有机体，这些细胞在接触肺部，由钛酸淀粉和电子显微镜提供。在阿维丁-生物素斯坦对细胞酮结构的研究中，他们有这样的精细中间膜作为氯胺酮和actin。这些结构是由不同的基底、特别是纤维素影响的。细胞的功能： Alveolar型<II>细胞在发生冲突后形成形式，他们认为它们是否有活跃的离子传输？滤波<II>器具有潜在差异的生物电子特性（PD）;-1.0 mV，电阻（R）; 200欧姆· &lt;cm 2&gt; ;短电路电流（SCC）; 1.8 uA/ &lt;cm 2&gt;。纤维涂层上的单体过滤器具有高于f上的PD和R的R ... More ilter。Taken together， fibronectin是一个重要的函数组件的extracellular矩阵，它支持alveolar类型<II>细胞在alveolar重复过程中。表面活性材料安全：澄清表面活性材料安全的机制，我们审查<beta>了表面活性材料安全的影响和&lt;Ca^（++）&gt;对表面活性材料安全的影响。一种肺损伤的动物模型：暴露85%的氧鼠具有甲状腺激素型细胞的增殖和超阳性<II>。我们试着找出一个因素，即什么是细胞增殖。从<II>遗留下来的人类腔型细胞的隔离。生活中的文化：如果这些细胞可能被损坏，表面接收器可能会被隔离过程和条件的某些方面丢失，那么我们将尝试在体内文化中开发一个新的系统，而隔离的细胞又被植入到不同程度的老年或次区域。示踪表皮细胞的功能：示踪表皮细胞有一些类似的功能，这些功能可以与Alveolar型<II>表皮细胞相似，例如离子传输、肌肉安全和繁殖。Less（低）

1. Cell isolation and culture: Alveolar type <II> cells were isolated from adult rat lungs by tissue dissociation with elastase and density gradient. The yield of the cells is about 20x <10^6> cells/rat and the purity of the adherent cells is 85-95 % type <II> cells. The cells appear similar lamellar bodies and organella to those in intact lung, by tannic acid stain and electron microscope. At the cytoskeleton structure examining by avidin-biotin stain, they have fine intermediate filaments such as keratin and actin. Those structure was affected by different substrates, especially fibronectin.2. Function of the cells: Alveolar type <II> cells form domes after confluent, which suggests that they have active ion transport. Alveolar type <II> monolayers on filter have bioelectronic properties of potential different(PD);-1.0 mV, resistance(R); 200 ohm・ <cm^2> ; short circuit current(SCC); 1.8 uA/ <cm^2> . The monolayers on fibronectin-coating filter have higher PD and R than those on the f … More ilter. Taken together, fibronectin is an important functional component of the extracellular matrix that supports alveolar type <II> cells during alveolar reepithelization.3. Surface active material secretion: To clarify the mechanism of surfactant secretion, we examined the effects of <beta> -stimulant and <Ca^(++)> on surfactant secretion.4. An animal model of lung injury: Exposure to 85 % oxygen rats had the proliferation and hypertrophy of alveolar type <II> cells. We have tried to find a factor which induces cell proliferation.5. Isolation of human alveolar type <II> cells from resected human lung.6. In vivo culture: Although the cells are necessarily damaged and lose surface receptors to some extent by the isolation procedure and conditions in vitro, we are trying to develop a new system of in vivo culture, which isolated cells are again implanted into the peritoneum or subcutaneous region of the different rat.7. Function of tracheal epithelium: The tracheal epithelial cells have some similar functions to those of alveolar type <II> epithelial cells, such as ion transport , mucus secretion and proliferation. Less