Molecular mechanisms of alveolar epithelial healing process in acute lung injury

急性肺损伤肺泡上皮愈合过程的分子机制

• 批准号: 23390376
• 负责人: SUGAHARA Kazuhiro
• 金额: $ 12.15万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23390376/
• 关键词: 急性肺傷害; 創傷治癒; 肺胞上皮細胞; 細胞増殖因子; EGFR; サイトカイン; NF-kB; EMT; エチルピルビン酸; epithelial-mesenchymal transition

1. We have demonstrated that 1) keratinocyte growth factor (KGF) is a potent factor for proliferation and differentiation of alveolar type II cells, 2) intratracheal instillation of KGF prevented acute lung injury by bleomycin and HCl. 2. Ethyl pyruvate reportedly has anti-inflammatory actions through inhibition of NF-kB. We confirmed that treatment of cultured alveolar epithelial cells with tumor necrosis factor a (TNFa) increased the NF-kB/DNA-binding activity, which was strongly inhibited with incubating with ethyl pyruvate. We also demonstrated that ethyl pyruvate interacts with RelA and p50 to inhibit their functions at multiple points. 3. Legionella pneumophila has flagella, causes pulmonary fibrosis. Microarray analysis in cultured alveolar epithelial cells indicated that flagellin increased fibronectin level and reduced E-cadherin level, which suggested that flagellin cooperates with TGF-b and induces EMT in alveolar epithelial cells, and causes fibrosis.

1.我们已经证明：1）角质细胞生长因子（KGF）是肺泡II型细胞增殖和分化的有效因子; 2）肺内滴注KGF可预防博莱霉素和盐酸所致的急性肺损伤。 2.据报道，丙酮酸乙酯通过抑制NF-κ B具有抗炎作用。 我们证实，治疗培养的肺泡上皮细胞与肿瘤坏死因子（TNF α）增加NF-κ B/DNA结合活性，这是强烈抑制与丙酮酸乙酯孵育。 我们还证明了丙酮酸乙酯与RelA和p50相互作用，在多个点抑制其功能。 3.嗜肺军团菌有鞭毛，导致肺纤维化。 培养的肺泡上皮细胞中的微阵列分析表明，鞭毛蛋白增加纤维连接蛋白的水平，降低E-cadherin的水平，这表明鞭毛蛋白与TGF-β协同作用，诱导肺泡上皮细胞EMT，导致纤维化。

项目成果

Noradrenaline does not impair postoperative renal function in endovascular stent graft repair

去甲肾上腺素不会损害血管内覆膜支架修复术后肾功能

• 发表时间: 2013
• 作者: T. Kinjo;Y. Madanbashi;S. Izumi;M. Oshiro;K. Sugahara
• 通讯作者: K. Sugahara

Skeletal muscle mass serves as an index of a neuromuscular junction blocking agent does

骨骼肌质量作为神经肌肉接头阻断剂的指标

• 发表时间: 2013
• 作者: H. Nishi;K. Kitano;S. Taira;M. Kakinohana;K. Sugahara
• 通讯作者: K. Sugahara

Noradrenaline does not impair postoperative renal function in endovascular stent graft repair.

去甲肾上腺素在血管内覆膜支架修复中不会损害术后肾功能。

• 发表时间: 2013
• 作者: T Kinjo;Y Madanbashi;S Izumi;M Oshiro;K Sugahara
• 通讯作者: K Sugahara

Skeletal muscle mass serves as an index of a neuromuscular junction blocking agent does.

骨骼肌质量充当神经肌肉接头阻断剂的指标。

• 发表时间: 2013
• 作者: H Nishi;K Kitano;S Taira;M Kakinohana;K Sugahara
• 通讯作者: K Sugahara

特集エビデンスに基づいた周術期の安全管理, 術中クライシスと外科的アプガースコア

特色：循证围手术期安全管理、术中危机和手术 Apgar 评分

• 发表时间: 2014
• 期刊: 麻酔
• 作者: 大城匡勝;須加原一博
• 通讯作者: 須加原一博