权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Development of the Rat Oral Vestibule --- The Physiological Cell Death in the Lip-furrow band.

大鼠口腔前庭的发育——唇沟带的生理细胞死亡。

基本信息

批准号：
60570845
负责人：
KINDAICHI Koji
金额：
$ 1.09万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570845/
关键词：
Lip-furrow band Oral vestibule Apoptosis Three-dimensionalaspect Personal-computer Electronmicroscope

项目摘要

The development of the oral vestibule were observed with light and electron microscopes. Three-dimensional aspects of the rat lip-furrow band obtained with the figurers drown from serial sections of mandibular parts by means of using cosmosone (Nikkon) for image analyser. Examined by light microscope, concurrently with the incisor dental lamina develops, the lip-furrow band forms independently, external to the dental laminas of left and right mandibularparts. Granular substances stained intensely with hematoxilen are clearly observed in the area of central of lip-furrow band on the 17th day of gestation. Subsequently, in those area, undifferential epithelial cells change into light, large and differentiated cells. Later, there are numerous keratohyalin granules in those areas symmetrically. Consequently, keratinized cells take the place of these cells. Finally, these central areas split and thereby the lip becomes separated from the gingiva and jaw. Examined by electron microscopy, varying in form and higher elecron density were nymerously scattered in or between the normal epithelial cells of the areas where the granular substance were observed by light microscopy. They were lost cell junction with neighbouring cells the cytoplasm became condensed and pyknotic nucleus. The split into serveral fragment, in which vacuoles, dilated cisteinae of rough endoplasmic reticulum, mitochondrial changes (swelling, lysis of cristae), dense bodies of irregular shapes were observed. They sere the characteristic feature of the apoptotic bodies. Finally, they were phagocytosed by neighbouring normal epithelial cells. It is assumed that there are relationship between the apoptosis and the cell differentiation.

用光学和电子显微镜观察口腔前庭的发育。使用 cosmosone (Nikkon) 图像分析仪，从下颌部分的连续切片中获得大鼠唇沟带的三维外观。通过光学显微镜检查，在切牙牙板发育的同时，唇沟带独立形成，位于左、右下颌部牙板的外部。妊娠第17天，在唇沟带中央区域可以清楚地观察到被苏木精染色的颗粒状物质。随后，在这些区域，未分化的上皮细胞变成轻的、大的和分化的细胞。随后，这些区域对称地出现大量透明角质颗粒。因此，角化细胞取代了这些细胞。最后，这些中心区域分裂，从而嘴唇与牙龈和下巴分离。通过电子显微镜观察，在光学显微镜观察到颗粒状物质的区域的正常上皮细胞内或之间散布着形状各异且电子密度较高的物质。它们失去了与邻近细胞的细胞连接，细胞质变得浓缩并且细胞核固缩。分裂成多个碎片，其中观察到空泡、粗面内质网半胱氨酸扩张、线粒体变化（嵴肿胀、溶解）、不规则形状的致密体。它们具有凋亡小体的特征。最后，它们被邻近的正常上皮细胞吞噬。推测细胞凋亡与细胞分化之间存在关系。