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Cytologic study of glandlar tumors of the oral region Diagnostic application of DNA flow cytometry -

口腔腺体肿瘤的细胞学研究 DNA 流式细胞术的诊断应用 -

基本信息

批准号：
60570923
负责人：
KAWASAKI Tateharu
金额：
$ 0.32万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570923/
关键词：
salivary gland tumor cytology nuclear DNA contents flow cytometry cell cycle cell cycle

项目摘要

Flow cytometric DNA measurements were performed on 6 salivary gland tumors, consisted of 1 benign pleomorphic adenoma, 2 adenoid cystic carcinomas, 1 acinic cell carcinoma, 1 malignant salivary gland tumor and 1 adenocarcinoma. Non-neoplastic controls consisted of 12 differrent specimens obtained from submandibular salivary glands, lymphonodes, and oral mucosal epitheliums. DNA ploidy and cell cycle distributions were calculated from DNA histograms and compared to cytologic data.Only 2 tumors ( 1 malignant salivary tumor and 1 adenocarcinoma) were clearly diagnosed as malignancy from their cytologic smears. But in the other 3 malignant tumors, cytologic diagnosis was very difficalt due to lack of cytologic malignant characteristics.In the DNA analysis, 4 of the 6 tumors (1 benign pleomorphic adenoma, 2 adnoid cystic carcinomas and 1 acinic cell carcinoma) showed 'nonmal' DNA diploid histograms such as seen in non-neoplastic control tissues. The DNA histogram of malignant salivary tumor had a diploid pattern with a significant increase in the tetraploid peak. The specimen of adenocarcinoma contained two types of tumor cells : a DNA diploid peak and a distinct DNA aneuploid peak. This tumor seemed to be a multiclonal type. The percentage of cells in S-phase (S-index) for all the diploid tumors were lower than for non-neoplastic controls. This finding indicated that the proliferantive activity of these tumors were lower than normal tissues. The S-index for the aneuploid tumor was simillar to the mean value for the non-neoplastic tissues.DNA analysis can offer distinctive tumor biological properties such as multiclonality and proliferent activity, and may yield clinically valuable information.

对 6 例唾液腺肿瘤进行流式细胞术 DNA 测量，其中包括 1 例良性多形性腺瘤、2 例腺样囊性癌、1 例腺泡细胞癌、1 例恶性唾液腺肿瘤和 1 例腺癌。非肿瘤对照由从下颌下唾液腺、淋巴结和口腔粘膜上皮获得的 12 个不同样本组成。根据DNA直方图计算DNA倍性和细胞周期分布，并与细胞学数据进行比较。只有2个肿瘤（1个恶性唾液肿瘤和1个腺癌）从细胞学涂片中明确诊断为恶性肿瘤。但在其他 3 种恶性肿瘤中，由于缺乏细胞学恶性特征，细胞学诊断非常困难。在 DNA 分析中，6 种肿瘤中的 4 种（1 例良性多形性腺瘤、2 例腺样囊性癌和 1 例腺泡细胞癌）显示出“非正常”DNA 二倍体直方图，如在非肿瘤对照组织中所见。唾液腺恶性肿瘤的DNA直方图呈二倍体模式，四倍体峰显着增加。腺癌标本含有两种类型的肿瘤细胞：DNA二倍体峰和独特的DNA非整倍体峰。该肿瘤似乎是多克隆型。所有二倍体肿瘤的 S 期细胞百分比（S 指数）均低于非肿瘤对照。这一发现表明这些肿瘤的增殖活性低于正常组织。非整倍体肿瘤的 S 指数与非肿瘤组织的平均值相似。DNA 分析可以提供独特的肿瘤生物学特性，如多克隆性和增殖活性，并可能产生有临床价值的信息。