Cytologic study of glandlar tumors of the oral region Diagnostic application of DNA flow cytometry -

对6例涎腺肿瘤进行了流式细胞术DNA检测，其中良性多形性腺瘤1例，腺样囊性癌2例，腺泡细胞癌1例，恶性涎腺肿瘤1例，腺癌1例。非肿瘤性对照包括12个不同的标本，取自颌下腺、淋巴结和口腔粘膜上皮。从DNA直方图计算DNA倍体和细胞周期分布，并与细胞学数据进行比较，仅2例肿瘤(1例恶性涎腺肿瘤和1例腺癌)的细胞学涂片明确诊断为恶性肿瘤。在DNA分析中，4例肿瘤(1例良性多形性腺瘤、2例腺样囊性癌和1例腺泡细胞癌)的DNA二倍体直方图与非肿瘤对照组织相同。涎腺恶性肿瘤DNA直方图呈二倍体模式，四倍体峰明显增多。腺癌标本含有两种类型的肿瘤细胞：DNA二倍体峰和明显的DNA非整倍体峰。该肿瘤似为多克隆型。所有二倍体肿瘤的S期细胞百分率(S指数)均低于非肿瘤对照组。这一发现表明，这些肿瘤的增殖活性低于正常组织。非整倍体肿瘤的S指数与非肿瘤组织的平均值相近。DNA分析可以提供独特的肿瘤生物学特性，如多克隆和增殖活性，并可能提供有临床价值的信息。

Flow cytometric DNA measurements were performed on 6 salivary gland tumors, consisted of 1 benign pleomorphic adenoma, 2 adenoid cystic carcinomas, 1 acinic cell carcinoma, 1 malignant salivary gland tumor and 1 adenocarcinoma. Non-neoplastic controls consisted of 12 differrent specimens obtained from submandibular salivary glands, lymphonodes, and oral mucosal epitheliums. DNA ploidy and cell cycle distributions were calculated from DNA histograms and compared to cytologic data.Only 2 tumors ( 1 malignant salivary tumor and 1 adenocarcinoma) were clearly diagnosed as malignancy from their cytologic smears. But in the other 3 malignant tumors, cytologic diagnosis was very difficalt due to lack of cytologic malignant characteristics.In the DNA analysis, 4 of the 6 tumors (1 benign pleomorphic adenoma, 2 adnoid cystic carcinomas and 1 acinic cell carcinoma) showed 'nonmal' DNA diploid histograms such as seen in non-neoplastic control tissues. The DNA histogram of malignant salivary tumor had a diploid pattern with a significant increase in the tetraploid peak. The specimen of adenocarcinoma contained two types of tumor cells : a DNA diploid peak and a distinct DNA aneuploid peak. This tumor seemed to be a multiclonal type. The percentage of cells in S-phase (S-index) for all the diploid tumors were lower than for non-neoplastic controls. This finding indicated that the proliferantive activity of these tumors were lower than normal tissues. The S-index for the aneuploid tumor was simillar to the mean value for the non-neoplastic tissues.DNA analysis can offer distinctive tumor biological properties such as multiclonality and proliferent activity, and may yield clinically valuable information.