Experimental studies on the injection of adriamycia into a third division of trigeminal nerve

三叉神经第三段注射阿霉素的实验研究

• 批准号: 10671895
• 负责人: KAWASAKI Tateharu
• 金额: $ 2.24万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671895/
• 关键词: Adriamycin; trigeminal nerve; trigeminal ganglion; retrograde axoplasmic transport

For the study according to the treatment of trigeminal neuralgia, we carried out the histological and electrophysiplogical study with injecting ADM to the trigeminal nerve terminal and transporting ADM to the ganglion by retrograde axoplasmic transport.1. Histological Study (1)Fluorescence microscopic Observation : We used female Wistar strain rats. Under general anesthesia (intraperitoneal injection of sodium pentobarbital), the inferior alveolar nerve was exposed at the mental foramen.10μ1 of a 5 %, 1 %, 0.5 %, 0.1 % concentration of ADM was injected into the nerve of each rat with a microsyringe. After 12 hours, 18 hours and 24 hours, the rat was perfused.The trigeminal ganglion was removed and quenched. 20 μm-thick sections were cut on a cryomicrotome.The specimens were examined with a fluorescence microscope to observe autofluorescence of ADM.. In result, 12 hours after 5 % ADM iniection, auto fluorescence of ADM was observed. And 18 hours after 1 % ADM mjection, weak auto fluores … More cence was observed. These showed ADM was transported to the trigeminal ganglion. (2)Histopathological findings : After injection of ADM, the trigeminal ganglion was removed (after 24 hours,7 days, and 21 days) and parafin-embedded.6 μm-thick sections were stained by the hematoxylin ana eosin, Kluver- Barrera methods.7 days after injection of 5 %ADM, ganglion cells showed vacuoles, chromatolysis, and displacement of nucleus.21 days after, perikaryon was dissolved and showed basket-like form. Gangion cells showed karyopyKnosis or karyoclasis.2. Electrophysiological study : The inferior alveolar nerve was exposed at the mental foramen : The nerve was stimulated by electric stimulation system and evoked potentials were recorded. Other rats were injected 5 % ADM to the nerve. After 24 hours, 7 days, and 21 days, the evoked potentials were recorded.24 hours after injection, evoked potentials were much lowwer than the potentials of no-injection rat. After 7days and 21days,the were form of evoked potentials were not recorded. Therefore, these results suggest that the method can destroy the trigeminal ganglion cells selectively. Less

本研究针对三叉神经痛的治疗，采用三叉神经末梢注射阿霉素（ADM）和逆行轴浆转运阿霉素（ADM）至三叉神经节的方法，进行了组织学和电生理学研究.组织学研究（1）荧光显微镜观察：选用雌性Wistar大鼠。在全身麻醉（腹腔注射戊巴比妥钠）下，于颏孔处暴露下牙槽神经，用微量注射器分别向神经内注射浓度为5%、1%、0.5%、0.1%的阿霉素10 μ l。12、18、24 h后灌流，取三叉神经节，冷处理。在冷冻切片机上切取20 μ m厚的切片，在荧光显微镜下观察ADM的自发荧光。结果，5%ADM注射后12小时，可见ADM自发荧光.注射1%ADM后18小时， ...更多信息 观察到了cence。结果表明，ADM被转运至三叉神经节。（2）组织学检查结果：注射ADM后，切除三叉神经节6 μ m厚切片行苏木精-伊红染色，Kluver-Barrera法。注射5%ADM后7天，神经节细胞出现空泡、染色质溶解和核移位。21天后，核周体溶解，呈篮状。神经节细胞核固缩或核碎裂.电生理学研究：在颏孔处暴露下牙槽神经，用电刺激系统刺激下牙槽神经，记录诱发电位。其余大鼠神经内注射5%ADM.注射后24 h、7 d、21 d记录诱发电位，注射后24 h诱发电位明显低于未注射组。7天和21天后，未记录到波形的诱发电位。因此，这些结果表明，该方法可以选择性地破坏三叉神经节细胞。少