A Basic Study on Treatment of Orthodontics Applied Sixth Component of Complement.

补体第六成分应用正畸治疗的基础研究。

• 批准号: 60570965
• 负责人: MORIYA Yoshiko
• 金额: $ 1.02万
• 依托单位: Nihon University School of Dentistry, at Matsudo.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570965/
• 关键词: 6th Component of Complement (C6); Prostaglandin E (PG E); カルシウム遊離

It has shown that the E Series of Prostaglandin (PG) Stimulate Bone Resorption and Increase the Adenyl Cyclase Activity in Bone and Bone Cell Membrene. In the Field of Orthodontics, it is Known that Orthodontic Force Caused an Increase the C AMP Levels.In this Study, We Investigated the Effects of PG <E_1> and Human 6th Component of Complement (C6) on the Release of <^(45)Ca> from Fetal Rat Calveria Previously Labeled with <^(45)Ca> in Organ Culture.The Resalts Were as Follows;1. <^(45)Ca> Release from Calveria Increased by the Addition of PG <E_1> to Culture Medium at Concentration of <10^(-8)> - <10^(-6)> M Dose Dependently.2. <^(45)Ca> Release Was Enhanced with Unheated Normal Human Serum, but with Heated Human Serum Only Slightly Enhanced.3. <^(45)Ca> Release was Stimurated by the Addition of 1.0 - 5.0 ug/ml Human Cl as Dose Dependent Fashion.4. By the Addition of Low Concentration of PG <E_1> ( <10^(-9)> M) or C6 (2.5 ug/ml) Alone Respectively, Ca Released Gradually, a Couple of Days after Adding.5. When PG <E_1> ( <10^(-9)> M) and C6 (2.5ug/ml)Were Added Together to Culture Medium, the Level of Released <^(45)Ca> Was Significantly Increased at Earler Time.In these Result, it Is Clear that PG <E_1> and Human Cl Have Synergistic Effect on Ca Release.

研究表明，E系列前列腺素（PG）刺激骨吸收，增加骨和骨细胞中腺苷酸环化酶的活性。在正畸学领域，正畸力可引起cAMP水平的升高，本研究探讨了PG和人<E_1>补体第六成分（C6）对体外培养的胎鼠胼胝体中标记的~（45）Ca释放的影响。通过<E_1>向培养基中添加浓度为&lt;10^（-8）&gt; - &lt;10^（-6）&gt; M的PG，从Calveria释放的&lt;^（45）Ca&gt;增加剂量依赖性。&lt;^（45）Ca&gt;释放在未加热的正常人血清中增强，但在加热的人血清中仅略微增强。通过添加1.0 - 5.0 μ g/ml人Cl刺激&lt;^（45）Ca&gt;释放，其为剂量依赖性方式。单独加入低浓度PG<E_1>（&lt;10^（-9）&gt; M）或C6（2.5 μ g/ml），细胞内钙释放量在加入后几天逐渐增加。当PG<E_1>（&lt;10^（-9）&gt; M）和C6（2.5 μ g/ml）同时加入培养液中时，早期释放的&lt;^（45）Ca&gt;明显增加，表明PG<E_1>和人Cl对Ca释放有协同作用。