Research on a peptide differentiation factor for myeloid leukemia cells

天然存在的分化因子由于其对宿主的低非特异性毒性而有望用于白血病治疗。我们发现从小鼠白血病M1细胞自发分化的细胞产生诱导M1细胞分化的低分子量因子。本研究建立了一种纯化该因子的方法。纯化方法包括Sephadex G-15凝胶过滤、阴离子交换色谱、薄层色谱、C18疏水载体反相HPLC。和HPLC凝胶过滤。氨基酸分析和由Rydon和Smith反应显色表明该因子是由12个氨基酸组成的肽。因子的<NH_2>-末端被阻塞。用纯化的因子诱导M1细胞分化为巨噬细胞样细胞。在5 × 10-^7&gt; μ g/ml浓度下，纯化的因子也抑制人白血病HL-60细胞的生长。

Naturally occurring differentiation factors are expected to be useful in leukemia therapy because of their low nonspecific toxicity to the host. we found cells spontaneously differentiated from mouse leukemia M1 cells were producing a low molecular weight factor that induces differentiation in M1 cells. In this study, we established a method to purify this factor. The procedure for purification includes gel filtration on Sephadex G-15, anionic exchange chromatography, thin-layer chromatography, reverse-phase HPLC on a C18 hydrophobic support. and HPLC gel filtration. Amino acid analysis and the color developed by the reaction of Rydon and Smith idicate that the factor is a peptide composed of 12 amino acids. The <NH_2> -terminal of the factor is blocked. M1 cells were induced to differentiatte into macrophage-like cells by the purified fctor. The purified factor at the concentration of 5 x <10-^7> g/ml also inhibits the growth of human leukemia HL-60 cells.