Research on a peptide differentiation factor for myeloid leukemia cells

髓系白血病细胞肽分化因子的研究

• 批准号: 60571058
• 负责人: NAKAYA Kazuyasu
• 金额: $ 0.9万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60571058/
• 关键词: Differentiation; Differentiation factor; Peptide; Leukemia cells; Peptide differentiation factor

Naturally occurring differentiation factors are expected to be useful in leukemia therapy because of their low nonspecific toxicity to the host. we found cells spontaneously differentiated from mouse leukemia M1 cells were producing a low molecular weight factor that induces differentiation in M1 cells. In this study, we established a method to purify this factor. The procedure for purification includes gel filtration on Sephadex G-15, anionic exchange chromatography, thin-layer chromatography, reverse-phase HPLC on a C18 hydrophobic support. and HPLC gel filtration. Amino acid analysis and the color developed by the reaction of Rydon and Smith idicate that the factor is a peptide composed of 12 amino acids. The <NH_2> -terminal of the factor is blocked. M1 cells were induced to differentiatte into macrophage-like cells by the purified fctor. The purified factor at the concentration of 5 x <10-^7> g/ml also inhibits the growth of human leukemia HL-60 cells.

由于自然发生的分化因子对宿主的非特异性毒性较低，因此有望在白血病治疗中发挥作用。我们发现从小鼠白血病M1细胞自发分化的细胞产生一种诱导M1细胞分化的低分子量因子。在本研究中，我们建立了一种纯化该因子的方法。纯化过程包括在Sephadex G-15上进行凝胶过滤，阴离子交换层析，薄层层析，在C18疏水载体上进行反相HPLC。高效液相色谱凝胶过滤。氨基酸分析和赖登和史密斯反应形成的颜色表明，该因子是由12个氨基酸组成的肽。因子的<NH_2> -端被阻塞。纯化后的因子诱导M1细胞向巨噬细胞样细胞分化。纯化后的因子浓度为5 × <10-^7> g/ml时，对人白血病HL-60细胞的生长也有抑制作用。

项目成果

Kazuyasu Nakaya, Noriko Kumakawa and Yasuharu Nakamura: "Partial purification and initial characterization of a novel differentiation factor for mouse myeloid leukemia cells" Biochemistry International. 10. 619-626 (1985)

Kazuyasu Nakaya、Noriko Kumakawa 和 Yasuharu Nakamura：“小鼠骨髓性白血病细胞新型分化因子的部分纯化和初步表征”生物化学国际。

Kazuyasu Nakaya, NorikoKumakawa, Hironobu Iinuma and Yasuharu Nakamura: "Purification of a low molecular weight factor which indues differentiation and inhibits growth in myeloid leukemia cells."

Kazuyasu Nakaya、NorikoKumakawa、Hironobu Iinuma 和 Yasuharu Nakamura：“纯化一种低分子量因子，可诱导骨髓性白血病细胞分化并抑制生长。”

中谷一泰: Biochemistry International. 10. 619-626 (1985)

中谷和康：《生物化学国际》10。619-626（1985）