Effects of isoprenoid compounds on human solid cancer cells

类异戊二烯化合物对人实体癌细胞的影响

基本信息

  • 批准号:
    09672251
  • 负责人:
  • 金额:
    $ 1.92万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1997
  • 资助国家:
    日本
  • 起止时间:
    1997 至 1998
  • 项目状态:
    已结题

项目摘要

We examined the effects of isoprenoid compounds such as geranylgeraniol and vitamin 1(2 or various solid cancer cell lines derived from human tissues. Geranylgeraniol induced apoptosis in every cancer cells examined in the present study. The 1C50 values range from 3O-9O muM.On the other hand, vitamin K2 that also contains a geranylgeranyl group in the molecule induced apoptosis only in two cell lines such as pancreatic cancer MIA-PaCa 2 cells and ovary cancer TYK-nu cells. When these cells were treated with vitamin K2, they shrank and small apoptotic bodies were formed 4 days after the incubation. Cycloheximide and actinomycin D inhibited the induction of apoptosis by vitamin K2, suggesting protein synthesis is necessary for apoptosis by vitamin K2. By contrast, the apoptosis induced by geranylgeraniol was not inbhibited by cycloheximide. In leukemia U937 cells, both JNK and caspase 3 were activated by the treatment with geranylgeraniol, while these enzymes were not activated by vitamin K2. These results suggest that geranylgeraniol and vitamin K2 might induce apoptosis by different mechanisms.
我们检测了类异戊二烯化合物如香叶基香叶醇和维生素1 β 2或来自人体组织的各种实体癌细胞系的作用。香叶基香叶醇在本研究中检测的每种癌细胞中诱导凋亡。1C 50值的范围为30 - 90 μ M。另一方面,在分子中还含有香叶基香叶基的维生素K2仅在两种细胞系如胰腺癌MIA-PaCa 2细胞和卵巢癌TYK-nu细胞中诱导凋亡。当这些细胞用维生素K2处理时,它们在孵育后4天收缩并形成小的凋亡小体。放线菌酮和放线菌素D抑制维生素K2诱导的细胞凋亡,表明蛋白质合成是维生素K2诱导细胞凋亡所必需的。放线菌酮对香叶基香叶醇诱导的细胞凋亡无明显抑制作用。在白血病U937细胞中,JNK和半胱天冬酶3被激活的治疗与香叶基香叶醇,而这些酶没有被激活的维生素K2。这些结果表明,香叶基香叶醇和维生素K2可能通过不同的机制诱导细胞凋亡。

项目成果

期刊论文数量(0)
专著数量(0)
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会议论文数量(0)
专利数量(0)
中谷一泰: "アポトーシスを標的とした治療薬" 医学のあゆみ. 187(5). 534-538 (1998)
Kazuyasu Nakatani:“针对细胞凋亡的治疗药物”,《医学史》187(5) (1998)。
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    0
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  • 通讯作者:
Yutaka Masuda et al.: "Geranylgeraniol potently induces caspase-3-like activity during apoptosis in human leukemia U937 cells." Biochem.Biophys.Res.Commun.234. 641-645 (1997)
Yutaka Masuda 等人:“香叶基香叶醇在人白血病 U937 细胞凋亡过程中有效诱导 caspase-3 样活性。”
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    0
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Yutaka Masuda, Mie Nakaya, Shigeo Nakajo, and Kazuyasu Nakaya.: "Geranylgeraniol potently induces CPP-32 like Protease activity during apoptosis in human Leukemia U937 cells." Biochem.Biophys.Res.Commun.234. 641-645 (1997)
Yutaka Masuda、Mie Nakaya、Shigeo Nakajo 和 Kazuyasu Nakaya:“香叶基香叶醇在人白血病 U937 细胞凋亡过程中有效诱导 CPP-32 样蛋白酶活性。”
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    0
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Toshihiro Aiuchi, Saori Mihara, Mie Nakaya, Yutaka Masuda, Shigeo Nakajo, and Kazuyasu nakaya.: "Zinc ions prevent processing of caspase-3 during apoptosis induced by geranylgeraniol in HL-60 cells." J.Biochem.124. 300-303 (1998)
Toshihiro Aiuchi、Saori Mihara、Mie Nakaya、Yutaka Masuda、Shigeo Nakajo 和 Kazuyasu nakaya:“在 HL-60 细胞中香叶基香叶醇诱导的细胞凋亡过程中,锌离子可阻止 caspase-3 的加工。”
  • DOI:
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  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Toshihiro Aiuchi et al.: "Zine Ions Prevent Processing of Caspase-3 during Apoptosis Induced by Geranylgeraniol in HL-60 Cells" J.Biochem.124. 300-303 (1998)
Toshihiro Aiuchi 等人:“在 HL-60 细胞中香叶基香叶醇诱导的细胞凋亡过程中,锌离子阻止 Caspase-3 的加工”J.Biochem.124。
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NAKAYA Kazuyasu其他文献

NAKAYA Kazuyasu的其他文献

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{{ truncateString('NAKAYA Kazuyasu', 18)}}的其他基金

High resolution analysis of proteins associated with the induction of cell death and carcinogenesis of tumor cells development of chemopreventive agents
与诱导细胞死亡和肿瘤细胞致癌相关的蛋白质的高分辨率分析 化学预防剂的开发
  • 批准号:
    15590068
  • 财政年份:
    2003
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of a novel anti-cancer agent from a novel tyrosine kinase inhibitor
从新型酪氨酸激酶抑制剂开发新型抗癌剂
  • 批准号:
    13672297
  • 财政年份:
    2001
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Studies on apoptosis-inducers targeted for the molecules associated with the induction of apoptosis in cancer cells
针对癌细胞凋亡诱导相关分子的凋亡诱导剂研究
  • 批准号:
    11672182
  • 财政年份:
    1999
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Combined effects of differentiation-apoptosis inducers on mice inoculated with human leukemia cells.
分化-凋亡诱导剂对接种人白血病细胞的小鼠的综合作用。
  • 批准号:
    06672239
  • 财政年份:
    1994
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
MECHANISM OF INDUCTION OF DIFFRENTIATION INLEUKEMIA CELLS BY TOPOISOMERASE INHIBITORS
拓扑异构酶抑制剂诱导白血病细胞分化的机制
  • 批准号:
    03671063
  • 财政年份:
    1991
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Research on a peptide differentiation factor for myeloid leukemia cells
髓系白血病细胞肽分化因子的研究
  • 批准号:
    60571058
  • 财政年份:
    1985
  • 资助金额:
    $ 1.92万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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  • 批准号:
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  • 财政年份:
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Elucidation of the action of geranylgeraniol which reciprocally controls bone resorption and bone formation and its application to osteoporosis
阐明香叶基香叶醇相互控制骨吸收和骨形成的作用及其在骨质疏松症中的应用
  • 批准号:
    16K11487
  • 财政年份:
    2016
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Biosynthesis of geranylgeranoic acid in animal cells
动物细胞中香叶基香叶酸的生物合成
  • 批准号:
    16K00862
  • 财政年份:
    2016
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Elucidating role of UBIAD1 in sterol-accelerated ERAD of HMG CoA reductase
阐明 UBIAD1 在 HMG CoA 还原酶甾醇加速 ERAD 中的作用
  • 批准号:
    8786245
  • 财政年份:
    2014
  • 资助金额:
    $ 1.92万
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Elucidation Role of UBIAD1 in Sterol-Accelerated ERAD of HMG CoA Reductase
阐明 UBIAD1 在 HMG CoA 还原酶甾醇加速 ERAD 中的作用
  • 批准号:
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  • 财政年份:
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阐明 UBIAD1 在 HMG CoA 还原酶甾醇加速 ERAD 中的作用
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