Fundamental study on mineral metabolism in race horses with bone fracture

骨折赛马矿物质代谢的基础研究

基本信息

批准号：
61440021
负责人：
OKADA Kosuke
金额：
$ 12.48万
依托单位：
Iwate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61440021/
关键词：
Horse Parathyroid gland Parafollicular cell Calcium Bone Fracture Parathormone Calcitonin 甲状腺傍瀘胞細胞骨折

项目摘要

1. Electron microscopic observation was performed on parathyroid and thyroid glands of 12 race-horses aged 3 to 9 years old which showed motor disturbances. The principal cells of the parathyroid gland were considered to be in the resting phase. On the other hand, the parafollicular cells of the thyroid gland were considered to be in the active phase. From 17 to 11,585 parafollicular cells were counted per 100 mm^2 of thyroid glandsections by immunoperoxidase method.2. Histopathological and biomechanical examinations were performed on bones from limbs of 40 horses suffering bone fracture in racing, and of 69 horses killed for other reasons. The dynamic stiffness and phase-lag (tan ) were measured by a viscoelastic spectrometer. It was suggested that the fractured sample was more brittle than the non-fractured sample.3. Paratholmone (PTH) and calcitonin (CT) levels in the serum were measured on 36 normal, 29 non-fractured and 17 fractured horses by radioimmnoassay. Unusual value were recorded in horses with fracture of large bone.4. Two g of calcium gluconate, 5g calcium chloride and 5g calcium bromide / day was administrated intravenously a horse. Although serum CT level rose highly following the injection, it dropped gradually during a following week. Normal level of PTH and CT were recorded in a horse which was administered same amount of calcium weekly for 16 times. This phenomenor might be due to homeostasis of the living body.5. Pathologic examination was performed on a case of systemic metastatic calcification in a race hose with bone fracture. Low phosphorus and normal calcium and magnesium levels in the serum and bone tissues were recorded. The systemic metastatic calcification might be resulted from nephrosis due to an excessive administration of vitamin D.6. From the results of the present examinations, some part of the mineral metabolism in horse with bone fracture were clarified.

1。对12至9岁的甲状旁腺和甲状腺腺体进行了电子显微镜观察，这显示出运动障碍。甲状旁腺的主要细胞被认为处于静止阶段。另一方面，甲状腺的副细胞被认为处于活性相。通过免疫过氧化物酶方法，从17至11,585个甲状腺肌脱落计数甲状腺分裂的17至11,585个。组织病理学和生物力学检查是在40匹马的四肢骨骼上进行骨折的骨折，并因其他原因而杀死的69匹马。通过粘弹性光谱仪测量动态刚度和相位滞后（TAN）。有人提出，断裂样品比未裂缝样品更脆。3。通过放射膜测定，在36个正常，29次非裂缝和17匹上的马匹上测量了血清中的Paratholmone（PTH）和降钙素（CT）水平。在大骨骨折的马中记录了异常价值。4。两克葡萄糖酸钙，5g氯化钙和5G溴化钙 /天静脉注射。尽管注射后血清CT水平高度上升，但在接下来的一周中逐渐下降。在每周相同数量的钙施用16次的马中记录了正常的PTH和CT水平。这种现象可能是由于活体的体内平衡引起的。5。在骨折的种族软管中，对全身转移性钙化的病例进行了病理检查。记录了血清和骨组织中低磷，正常钙和镁水平。全身转移性钙化可能是由于维生素D.6的过量施用引起的肾脏症。从当前检查的结果中，阐明了马骨折的矿物质代谢的某些部分。