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Fundamental study on mineral metabolism in race horses with bone fracture

骨折赛马矿物质代谢的基础研究

基本信息

批准号：
61440021
负责人：
OKADA Kosuke
金额：
$ 12.48万
依托单位：
Iwate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61440021/
关键词：
Horse Parathyroid gland Parafollicular cell Calcium Bone Fracture Parathormone Calcitonin 甲状腺傍瀘胞細胞骨折

项目摘要

1. Electron microscopic observation was performed on parathyroid and thyroid glands of 12 race-horses aged 3 to 9 years old which showed motor disturbances. The principal cells of the parathyroid gland were considered to be in the resting phase. On the other hand, the parafollicular cells of the thyroid gland were considered to be in the active phase. From 17 to 11,585 parafollicular cells were counted per 100 mm^2 of thyroid glandsections by immunoperoxidase method.2. Histopathological and biomechanical examinations were performed on bones from limbs of 40 horses suffering bone fracture in racing, and of 69 horses killed for other reasons. The dynamic stiffness and phase-lag (tan ) were measured by a viscoelastic spectrometer. It was suggested that the fractured sample was more brittle than the non-fractured sample.3. Paratholmone (PTH) and calcitonin (CT) levels in the serum were measured on 36 normal, 29 non-fractured and 17 fractured horses by radioimmnoassay. Unusual value were recorded in horses with fracture of large bone.4. Two g of calcium gluconate, 5g calcium chloride and 5g calcium bromide / day was administrated intravenously a horse. Although serum CT level rose highly following the injection, it dropped gradually during a following week. Normal level of PTH and CT were recorded in a horse which was administered same amount of calcium weekly for 16 times. This phenomenor might be due to homeostasis of the living body.5. Pathologic examination was performed on a case of systemic metastatic calcification in a race hose with bone fracture. Low phosphorus and normal calcium and magnesium levels in the serum and bone tissues were recorded. The systemic metastatic calcification might be resulted from nephrosis due to an excessive administration of vitamin D.6. From the results of the present examinations, some part of the mineral metabolism in horse with bone fracture were clarified.

1.对12匹3 ~ 9岁运动障碍赛马的甲状旁腺和甲状腺进行了电镜观察。认为甲状旁腺的主要细胞处于静息期。另一方面，甲状腺滤泡旁细胞被认为处于活跃期。免疫过氧化物酶法每100 mm^2甲状腺切片计数滤泡旁细胞17 ~ 11，585个.本文对40匹赛马中发生骨折的马和69匹因其他原因死亡的马的四肢骨进行了组织学和生物力学的检查。动态刚度和相位滞后（tan）由粘弹性光谱仪测量。这表明，断裂试样比未断裂试样更脆.用放射免疫法测定了36匹正常马、29匹非骨折马和17匹骨折马血清甲状旁腺激素（PTH）和降钙素（CT）的含量。在大骨骨折的马中记录了异常值。马每天静脉注射葡萄糖酸钙2g、氯化钙5g和溴化钙5g。血清CT水平在注射后迅速升高，但在随后的一周内逐渐下降。每匹马每周给予等量钙剂16次，观察其PTH和CT的正常值。这种现象可能是由于生物体的内稳态.本文报告一例全身转移性钙化并骨折的病例。记录血清和骨组织中的低磷和正常钙和镁水平。全身转移性钙化可能是由于过量服用维生素D.6引起的肾病。从目前的检查结果中，阐明了骨折马矿物质代谢的某些部分。