Role of tumor-associated, phosphorylated glycoprotein-membrane-antigen in bovine leukemia virus-induced lymphosarcoma.

肿瘤相关磷酸化糖蛋白膜抗原在牛白血病病毒诱导的淋巴肉瘤中的作用。

基本信息

批准号：
05404016
负责人：
OKADA Kosuke
金额：
$ 16.7万
依托单位：
Iwate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1996
项目状态：
已结题

项目摘要

Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis (EBL), which is the most common neoplastic disease of cattle. To clarify the way in which BLV-infected cattle progress from the asymptomatic stage to the lymphoma stage, we produced a monoclonal antibody (Mab) c143 which recognized a tumor-associated antigen (TAA) that is phosphorylated in the transformed state of BLV-infected B-lymphoid cells. The TAA was mainly expressed on B-cells, macrophages, reticular cells, and a minor population of BoCD4-positive T-cells in BLV-free normal cattle. Since the nature of c143 TAA was likely to be that of the major histocompatibility complex (MHC) class II antigens, we isolated cDNAs for bovine MHC (BoLA) class alpha-chains (36-37 kD) and beta-chains (32 and 34 kD), produced transfectants that expressed a single type of BoLA class II molecules and analyzed them by flow cytometry with c143 Mab. The c143 Mab recognized the transfectant expressing BoLA-DR but not BoLA-DQ.However, the treatment of lymphocytes with c143 or anti-BoLA-DR Mab induced different effects. Although mixed lymphocyte reaction (MLR) was inhibited by the addition of anti-BoLA-DR Mab, the c143 Mab did not inhibit a proliferative response of T cells in MLR.Increased spontaneous proliferation of lymphocytes in healthy donors was obtained in the presence of c143 Mab but not anti-BoLA-DR Mab, and was much in lymphocytes from the carrier. More over, the patterns of immunohistological staining for c143 Mab in BLV-infected sheep showed distinguishing differences from those of anti-BoLA-DR Mabs.An immunohistochemical examination using c143 Mab as tumor marker for identification of EBL indicated that the bovine leukemia virus-transformed lymphocytes or neoplastic cells in peripheral blood accumulate in the marginal sinus area at the earliest stages, and subsequently proliferate and infiltrate into follicles, leading to the development of clinical signs of lymphosarcoma.

牛白血病病毒（BLV）与牛牛白细胞病（EBL）有关，这是牛最常见的肿瘤疾病。为了阐明BLV感染的牛从无症状阶段到淋巴瘤阶段的发展方式，我们产生了一种单克隆抗体（MAB）C143，该抗体C143识别出肿瘤相关的抗原（TAA），该抗原（TaA）在BLV感染的B-淋巴细胞转化状态下磷酸化。 TAA主要以B细胞，巨噬细胞，网状细胞和少量的BOCD4阳性T细胞在无BLV的正常牛中表达。 Since the nature of c143 TAA was likely to be that of the major histocompatibility complex (MHC) class II antigens, we isolated cDNAs for bovine MHC (BoLA) class alpha-chains (36-37 kD) and beta-chains (32 and 34 kD), produced transfectants that expressed a single type of BoLA class II molecules and analyzed them by flow cytometry with C143 mab。 C143 mAb识别表达bola-dr但不表达bola-dq的转染剂。 Although mixed lymphocyte reaction (MLR) was inhibited by the addition of anti-BoLA-DR Mab, the c143 Mab did not inhibit a proliferative response of T cells in MLR.Increased spontaneous proliferation of lymphocytes in healthy donors was obtained in the presence of c143 Mab but not anti-BoLA-DR Mab, and was much in lymphocytes from the 载体。 More over, the patterns of immunohistological staining for c143 Mab in BLV-infected sheep showed distinguishing differences from those of anti-BoLA-DR Mabs.An immunohistochemical examination using c143 Mab as tumor marker for identification of EBL indicated that the bovine leukemia virus-transformed lymphocytes or neoplastic cells in peripheral blood accumulate in the marginal鼻窦区域最早的阶段，随后扩散并浸润到卵泡中，导致淋巴瘤临床体征的发展。