Axenic culture of Japanese radish-downy mildew fungus

日本萝卜霜霉病菌的无菌培养

• 批准号: 61560058
• 负责人: OHGUCHI Tomizo
• 金额: $ 1.47万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61560058/
• 关键词: Downy Mildew Fungus Of Japanese Radish; Obligate Parasite; Axenic Culture; 病害抵抗性; 抵抗性

Comparing behavior of downy mildew fungus in diseased tissues between two Japanese radish cultivars different in resistance revealed that although growth of the fungus was remarkably different in speed, no clear difference in morphology of intercellular hypha and haustorium was not observed under a light microscope.In order to get axenic culture of this fungus the growth on various media was investigated by using conidia and infected slices as starting materials. While Williams's medium used for successful axenic culture of Puccinia graminis, althogh added various nutrients, was ineffective, Knop's medium promoted the growth of germ-tube. Homogenate of root of Japanese radish inhibited germination of conidium strikingly. However, the homogenate dialized against distilled water and also fraction 5 of it, fractionated by Sephadex G-100 stimulated growth of germ-tube considerably. On various membrances, especially epidermis of scale leave of onion germed conidia formed appressoria and infection hyphae. The infection hyphae grew in intercellular space of the epidermis cell but could not grow on the medium. Many haustorium-like structures were produced on Knop's medium when infected slices were used. Addition of yeast and liver extract, casein hydrate, cod liver oil, minerals and liver to the Knop's medium did not promote formation of the fungal organ. Growth of the fungus was stimulated on the dialized homogenate-medium and also the fraction 5-medium. Numbers of hypha and haustorium-like structure, and length of hypha were increased. Decoction of cell wall- and fluid of intercellular space of root tissue-media also stimulated formation of haustorium-like structure. The former stimulated growth of hypha especially.As mentioned above, this fungus formed all organs which observed in the diseased tissue, on some Japanese radish components-media. Bur the term of growth was short. All efforts to elongate it were not successful.

比较霜霉病菌在两个抗病性不同的日本萝卜品种病株组织中的生长行为，结果表明，虽然霜霉病菌在病株组织中的生长速度有显著差异，但在光镜下观察到的胞间菌丝和吸器形态无明显差异。威廉姆斯培养基虽添加了多种营养物质，但对小麦柄锈菌无菌培养效果不佳，而Knop培养基则促进了芽管的生长。日本萝卜根匀浆对分生孢子萌发有明显的抑制作用。而经蒸馏水透析的匀浆液和SephadexG-100分级分离的组分5对芽管的生长有明显的促进作用。在洋葱鳞片叶的不同膜上，特别是表皮上，萌发的分生孢子形成附着胞和侵染菌丝。侵染菌丝生长于表皮细胞间隙，不能在培养基上生长。当使用感染的切片时，在Knop培养基上产生许多吸器样结构。添加酵母和肝脏提取物，酪蛋白水合物，鱼肝油，矿物质和肝脏的诺普培养基没有促进真菌器官的形成。真菌的生长在透析的匀浆培养基和级分5培养基上刺激。菌丝数、吸器样结构数和菌丝长度增加。根组织培养基的细胞壁和细胞间隙液也促进吸器样结构的形成。前者对菌丝生长有明显的促进作用，在日本萝卜的某些成分培养基上，该菌形成了病组织中观察到的所有器官。但增长期较短。所有延长它的努力都没有成功。

项目成果

Tomizo Ohguchi: "Morphology of intercellular hyphae of downy mildew fungus in Japanese radish" Ann. Phytopath. Soc. Japan.

Tomizo Ohguchi：“日本萝卜霜霉病菌细胞间菌丝的形态”安。

Tomizo Ohguchi: "Behavior of downy mildew fungus of Japanese radish on media" Ann. Phytopath. Soc. Japan.

Tomizo Ohguchi：“媒体上的日本萝卜霜霉病菌的行为”安。