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Taxonomical and ecological study of the gall disease bacteria isolated from the trees used for dyeing of Oshima and Kumejima tsumugi textiles.

从用于大岛和久米岛 tsumugi 纺织品染色的树木中分离出的瘿病细菌的分类学和生态学研究。

基本信息

批准号：
61560175
负责人：
OGIMI Choei
金额：
$ 1.09万
依托单位：
University of the Ryukyus
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

A new bacterial disease with gall formation on either trunks or twigs was found on Sharinbai(Rhaphiolepis umbellate Makino) trees growing in Okinawa and Kagoshima Prefecture, Japan. The surface of young galls was relatively smooth and light-green in color, but they became rough and light-brown to dark-brown with age. A pathogenic bacterium isolated from these galls was gram-negative rod with 1 to several polar flagella, aerobic and metabolized glucose oxidatively. Negative results were obtained with the tests such as kovacs oxidase, levan production, potato soft rot, hydrolysis of esculin and starch, arginine dihydrolase, production of H_2S, nitrate reduction, KCN inhibition, indole production, gelatin liguefaction, methyl-red and Voges-Proskauyer tests and growth at 41゜C and 4゜C. Positive results were obtained with the tests such as catalase, tobacco hypersensitive reaction, hydrolysis of Tween 80, reducing substances from sucrose and fluorescent pigment production. Following organic compounds were utilized as a sole source of carbon : ribose, glucose, D-mannose, D-xylose, fructose, glycerol, D-sorbitol, mannitol, citrate, succinate, L-malate and L-, meso-tartrate. Followings were not utilized : sucrose, D-,L-arabinose, L-rhamnose, Dk-galactose, lactose, maltose, D-cellobiose, melibiose, D-trehalose, dextrin,glycogen, maltose, starch, inulin, inositol, adonitol, dulcitol, salicin, malonate, lactate, L-Valine, L-Arginine and <beta>-Alanine. Inoculation tests for the host range of the Sharinbai pathogen were conducted on trees of 105 species belonging to 44 families. The disease development was confirmed only on Rhaphiolepis umbellata, but not on other trees, From these results, the Sharinbai pathogen was identified as a new pathovar of Pseudomonas syringae. The name of P. syringae pv. rhaphiolepidis Pv. nov. will be soon proposed to ATCC, NCPPB, PDDCC and the strain RU 2 will be designated as the pathotype strain.

在日本冲绳和鹿儿岛县发现了一种新的细菌性病害，该病害可在树干或枝条上形成虫瘿。幼嫩虫瘿表面相对光滑，颜色淡绿，但随着年龄的增长，表面变得粗糙，呈浅褐色至深褐色。从这些虫瘿中分离到一株革兰氏阴性杆菌，有1 ~数根极生鞭毛，需氧，葡萄糖氧化代谢。在41 ℃和4 ℃条件下，Kovacs氧化酶、莱万生成、马铃薯软腐病、七叶苷和淀粉水解、精氨酸二氢酶、H_2S生成、硝酸盐还原、KCN抑制、吲哚生成、明胶液化、甲基红和Voges-Proskauyer试验以及生长试验均为阴性。经过氧化氢酶、烟草过敏反应、吐温80水解、蔗糖还原物、荧光色素生成等试验，均获得阳性结果。以下有机化合物用作唯一的碳源：核糖、葡萄糖、D-甘露糖、D-木糖、果糖、甘油、D-山梨糖醇、甘露糖醇、柠檬酸盐、琥珀酸盐、L-苹果酸盐和L-内消旋酒石酸盐。未使用以下内容：蔗糖、D-、L-阿拉伯糖、L-鼠李糖、Dk-半乳糖、乳糖、麦芽糖、D-纤维二糖、蜜二糖、D-海藻糖、糊精、糖原、麦芽糖、淀粉、菊粉、肌醇、侧金盏花醇、卫矛醇、水杨苷、丙二酸盐、乳酸盐、L-缬氨酸、L-精氨酸和<beta>β-丙氨酸。对44科105种树木进行了沙林白病菌寄主范围的接种试验。结果表明，沙林白病菌是一种新的病原菌，其致病性仅在漏芦上得到证实，而在其它树种上未发现。P. pv.的名称。Rhaphiolaea是Pv. nov.将很快向ATCC、NCPPB、PDDCC提出，并将菌株RU 2指定为致病型菌株。