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Taxonomical and Ecological Study of the Cancerous Disease Bacteria from Nakaharakuroki (Symplocos lucida var. nakaharae Mak. & Nemoto)

Nakaharakuroki 癌病细菌（Symplocos lucida var. nakaharae Mak.）的分类学和生态学研究

基本信息

批准号：
01560173
负责人：
OGIMI Choei
金额：
$ 1.6万
依托单位：
University of the Ryukyus
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

A new bacterial disease with cancer formation on either trunks, twigs or petioles was found on nakaharakuroki (Symplocos lucida var. nakaharae Mak. & Nemoto) in Okinawa prefecture, Japan. The surface of galls was bump or cancer and from light brown to brown in color. A pathogenic bacterium isolated from these cancers was a gram negative rod with 1-5 polar flagella ; PHB was accumulated ; aerobic and metabolized glucose oxidatively. Maximum, optimum and minimum growth temperatures were 38^ﾟC, 29^ﾟC-35^ﾟC and 15^ﾟC. Growth took place at pH of 4.0 to 7.0. Negative results were obtained in the tests such as indol production, arginine dihydrolase, production of reducing substance from sucrose, zelatin liquefaction, gluconate oxidation, tobacco H. R., tween80 hydrolysis, levan production and growth at 41^ﾟC and 4^ﾟC. Sucrose, melibiose, dextrin, starch, adonitol, D-, L-tartaric acid and L-valine were not utilized as sole source of carbon. lt responded positively to the tests of catalase and Kovacs oxidase reaction, nitrate reduction, H_2S production, denitrification, urease production, tyrosinase test and ammonia production. D-ribose, D-, L-arabinose, L-rhamnose, D-mannose, sodium lactate, L-alpha-alanine, L-arginine and beta-alanine were utilized as sole source of carbon. Inoculation tests for the host range of the nakaharakuroki pathogen were conducted on 178 species of trees and plants belonging to 65 families. Outbreaks of the disease were observed on nakaharakuroki (S. l. var. n.), kuroki (S. l.) and tobacco (Nicotiana tabaccum L.) but not on other trees and plants. From these results the nakaharakuroki pathogen was identified as a new species of Pseudomonads. From the characteristic symptoms mentioned above "Cancerous bacteria disease of nakaharakuroki" was proposed as the new disease name. The name of Pseudomonas symploci n. sp. will be soon deposited in ATCC, NCPPB and ICMP and the strain SLV3 will be designated as the type strain.

摘要在中原木（Symplocos lucida var. nakaharae Mak）上发现了一种树干、小枝或叶柄均可癌变的细菌性病害。& Nemoto)，日本冲绳县。胆囊表面肿块或癌状，颜色由浅棕色变为褐色。从这些癌症中分离出的致病菌为革兰氏阴性杆状体，有1-5个极性鞭毛；PHB积累；有氧和氧化代谢葡萄糖。最高、最适和最低生长温度分别为38^ C、29^ C-35^ C和15^ C。生长在pH为4.0 ~ 7.0的环境中。在41^ C和4^ C条件下，吲哚生成、精氨酸二水解酶、蔗糖生成还原性物质、zelatin液化、葡萄糖酸盐氧化、烟草H. R、tween80水解、levan生成和生长等试验均获得阴性结果。蔗糖、蜜二糖、糊精、淀粉、甘油三醇、D-、l -酒石酸和l -缬氨酸不是碳的唯一来源。对过氧化氢酶和Kovacs氧化酶反应、硝酸盐还原、H_2S生成、反硝化、脲酶生成、酪氨酸酶生成和氨生成试验均有较好的反应。D-核糖、D-、l -阿拉伯糖、l -鼠李糖、D-甘露糖、乳酸钠、l - α -丙氨酸、l -精氨酸和β -丙氨酸是碳的唯一来源。对我国65科178种树木和植物进行了中原栗病寄主范围的接种试验。在中原木（s.l. var. n.）、黑木（s.l.）和烟草（Nicotiana tabaccum l.）上观察到该病害的暴发，但在其他树木和植物上未见暴发。根据这些结果，中原栗病菌被确定为假单胞菌的一个新种。从上述特征症状出发，提出了“癌性细菌病”作为新的疾病名称。拟在ATCC、NCPPB和ICMP上沉积假单胞菌（Pseudomonas symploci n. sp.）的名称，并将菌株SLV3指定为型菌株。