Isolation of the first component (C1) and the second component (C2) of carp complement

采用6步纯化法从鲤鱼血清中分离出补体第一组分（C1）。从300 ml鲤鱼血清中可得到约2.8 mg鲤鱼氯。纯化后的白藜芦醇苷的溶血活性和血清蛋白含量分别为18%和0.023%，纯化倍数为788倍。用抗鲤鱼全血清（兔）免疫电泳法，确定了高纯度的鲤鱼Cl。鲤鱼C1的分子量估计为1，020，000。EDTA处理可抑制鲤鱼C1的溶血活性，但用含Ca^2+的缓冲液透析可克服这种抑制作用。鲤鱼C_1的溶血活性可被加热（50 ℃，15分钟）或与角叉菜胶孵育破坏，但与氨、肼或酵母聚糖孵育时仍能保持。这些结果表明，鲤鱼C1的化学性质类似于哺乳动物C1。通过孵育EAC 1，4（Yano等人，1986）在Mg^<2+>存在下，用肼处理的鲤鱼血清（Hd），其缺乏C3。用不同的灭活血清进行试验，结果表明EAC 1，4，2与EAC 1，4有明显的差异。还证实了EAC 1，4，2和C3之间的反应在没有任何二价阳离子的情况下进行。作者目前正在努力从鲤鱼血清中分离C2。数据将在不久的将来公布。

The first component of complement(C1) was isolated from carp serum by a 6-step purification procedure. About 2.8 mg of carp Cl was obtained from 300 ml of carp serum. The final preparatin contained 18% of the initial Cl hemolytic activity and 0.023% of the initial serum prote in, representing 788-fold purification. The high purity of the isolated carp Cl was established by immunoelectrophoresis against anti-whole carp serum (rabbit). The molecular weight of carp C1 was estimated to be 1,020,000. The hemolytic activity of carp C1 was inhibited by EDTA treatment, but this inhibition was overcome by dialysis against a Ca^<2+>-containing buffer. The hemolytic activity of carp C1 was destroyed by heating (50 C, 15 min) or incubation with carrageenan, but was retained when incubated with ammonia, hydrazine or zymosan. These results indicate that carp C1 resembles mammalian C1 in its chemical properties. An intermediate complex EAC1,4,2 was prepared by incubating EAC1,4 (Yano et al., 1986) with hydrazine treated carp serum (Hd), which is deficient in C3, in the presence of Mg^<2+>. The difference between EAC1,4,2 and EAC1,4 was shown by the tests with various inactivated sera. It was also confirmed that the reaction between EAC1,4,2 and C3 proceeds without any divalent cation. The author is now making efforts to isolate C2 from carp serum. Data will be published in the near future.