Molecular cloning and expression analysis of acute phase reactants in bony fish.

用抑制性消减杂交技术克隆了鲤鱼和虹鳟鱼急性期反应物的互补DNA。刺激剂包括海藻酸钠、松节油、硬葡聚糖和福尔马林灭活鳗弧菌。因此，从鲤鱼中鉴定出约50个基因，包括哺乳动物中已知的急性时相蛋白，如五肽和血清淀粉样蛋白A，以及几种趋化因子、趋化因子受体、新的C型凝集素、新的补体因子B亚型、胶质细胞成熟因子、IL-1β和前B细胞增强因子。另一方面，从鲑鱼肝中鉴定出36个基因，包括LECT2趋化蛋白、补体B/C2、C型凝集素、纤维蛋白原、结合珠蛋白、血凝素、CD59、溶菌酶、维他命原、前血小板蛋白、Toll样受体、转铁蛋白、血清淀粉样蛋白A、C-多糖结合蛋白和凝血酶。有趣的是，在哺乳动物中结构性表达的补体成分B/C2和CD59在硬骨鱼中的表达水平显著增加，这表明一些补体成分在硬骨鱼中是急性期反应物。

Complementary DNAs encoding acute phase reactants in the common carp and rainbow trout were cloned by means of the suppression subtractive hybridization. Sodium alginate, turpentaine oil, scleroglucan, and formaline-killed Vibrio anguillarum were used as stimulants. As a result, about 50 genes including acute phase protein known in mammals, such as pentraxin and serum amyloid A, as well as several chemokines, chemokine-receptors, a novel C-type lectin, a novel complement factor B isotypes, glia-maturation factor, IL-1beta, and pre-B cell enhancing factor were identified from carp. On the other hand, 36 genes including LECT2 chemotaxin, complement B/C2, a C-type lectin, fibrinogen, haptoglobin, hemopexine, CD59, lysozyme, viterogenin, precelebrin, Toll-like receptors, transferrin, serum amyloid A, C-polysaccharide-binding protein and thrombin were identified from trout liver. It is intriguing that complement components such as factor B/C2 and CD59, which are expressed constitutively in mammals, showed remarkable increase in their expression levels in response to the stimuli in bony fish, suggesting that some complement components are acute phase reactant in bony fish.