Production of antiserum against pyrogenic factor and pyrogenic mechanisms using the antiserum.

近年来研究发现，免疫应答与中枢神经系统在宿主防御激活中的作用密切相关。发热反应是由白细胞介素-1（IL-1）介导的，IL-1还激活免疫和炎症反应，并在血脑屏障内外复杂地处理。为探讨发热的详细机制，本研究利用白细胞介素1（IL-1）单克隆抗体制备了抗内源性热原（EP）的单克隆抗体。本实验最终获得的抗EP粗品单克隆抗体本身具有致热原性。在本实验之后，虽然使用了重组IL-1作为抗原，但我们未能引入无致热原性的单克隆抗体<alpha>，我们恰好有针对人重组IL-1或IL-1的多克隆抗体<alpha><bet>。脑室注射抗体时，家兔直肠温度无变化，而静脉注射抗体与IL-1的混合物引起发热反应。icv注射抗IL-1<bet>抗体可消除静脉注射大量IL-1所引起的双相热晚期<beta>。然而，icv注射抗IL-1抗体<alph>并不能消除大量IL-1给药的双相热的任何阶段<alph>。提示血脑屏障内出现的致热因子可能是IL-1<beta>。

It has recently been revealed that immune response are closely related to the central nervous system in activation of host defense. Fever response was mediated by Interleukin-1 (IL-1) which also activated both immune and inflammatory responses and was complicatedly processed outside or inside the blood-brain barrier. To investigate detail mechanisms of fever production, using antibody against Interleukin-1, we tryed to make monoclonal antibody against crude endogenous pyrogen (EP). Monoclonal antibody against crude EP achieved finally in our experiment showed pyrogenic nature by itself. Next to this experiment we failed to introduce monoclonal antibody without pyrogenicity although recombinant IL-1<alpha> was used as antigen.We happen to have had polyclonal antibody against human recombinant IL-1<alpha> or IL-1<bet>. When the antibody was intraventricularly injected no change was observed in rectal temperature of rabbits, while iv infusion of a mixture of antibody with IL-1 caused febrile response. Then icv injection of antibody ageinst IL-1<bet> abolished the late pase of biphasic fever necessarrily induced following by iv injection of a large amount of IL-1]<beta>. However icv injection of antibody against IL-1<alph> did not abolish any phase of biphasic fever with a large amount of IL-1<alph> administration. This may be a fact suggesting that pyrogenic factor appeared insigde the BBB must be IL-1<beta>.