Production of antiserum against pyrogenic factor and pyrogenic mechanisms using the antiserum.

最近研究发现，免疫反应与中枢神经系统在激活宿主防御中密切相关。发热反应是由白介素1(IL-1)介导的，它还激活免疫和炎症反应，并在血脑屏障外或内复杂地进行。为了探讨发热产生的具体机制，我们利用白介素1抗体制备了抗内源性热原(EP)的单抗。本实验最终获得的抗EP粗品的单抗本身具有致热性。下一步，我们没有引入不具有致热性的单抗，虽然我们使用了重组IL-1α&gt；作为抗原，我们碰巧有抗人重组IL-1α&gt；或IL-1；t；bet&gt；的多克隆抗体。抗体侧脑室注射对兔直肠温度无明显影响，静脉滴注抗体与IL-1混合液可引起发热反应。然后，侧脑室注射抗IL-1的抗体，消除静脉注射大量IL-1、β和gt；所致的双相发热。然而，侧脑室注射抗IL-1α抗体并不能消除双相发热的任何阶段，同时大量注射IL-1α。这可能是一个事实，提示出现了致热因子，提示血脑屏障一定是IL-1β。

It has recently been revealed that immune response are closely related to the central nervous system in activation of host defense. Fever response was mediated by Interleukin-1 (IL-1) which also activated both immune and inflammatory responses and was complicatedly processed outside or inside the blood-brain barrier. To investigate detail mechanisms of fever production, using antibody against Interleukin-1, we tryed to make monoclonal antibody against crude endogenous pyrogen (EP). Monoclonal antibody against crude EP achieved finally in our experiment showed pyrogenic nature by itself. Next to this experiment we failed to introduce monoclonal antibody without pyrogenicity although recombinant IL-1<alpha> was used as antigen.We happen to have had polyclonal antibody against human recombinant IL-1<alpha> or IL-1<bet>. When the antibody was intraventricularly injected no change was observed in rectal temperature of rabbits, while iv infusion of a mixture of antibody with IL-1 caused febrile response. Then icv injection of antibody ageinst IL-1<bet> abolished the late pase of biphasic fever necessarrily induced following by iv injection of a large amount of IL-1]<beta>. However icv injection of antibody against IL-1<alph> did not abolish any phase of biphasic fever with a large amount of IL-1<alph> administration. This may be a fact suggesting that pyrogenic factor appeared insigde the BBB must be IL-1<beta>.