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Biochemical studies on the pathogenetic mechanism of toxic neuropathies by using axonal transport.

利用轴突运输对中毒性神经病发病机制进行生化研究。

基本信息

批准号：
61570136
负责人：
KOMIYA Yoshiaki
金额：
$ 1.47万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

Various neurotoxic chemicals are thought to be classified into several groups according to their main targets. Among them three major groups were chosen and in vivo model systems were developed to investigate their biochemical mechanism of action. By analysing slow axonal transport under the influence of these drugs, the following results were obtained.u. Neurotoxicity of colchicine:Colchicine completely and exclusively blocks tubulin transport, without affecting that of actin and neurofilament proteins. when 4 g of colchicine is injected into fifth lumbar dorsal root ganglion of 7 week-old rat, almost all unmyelinated axons as well as a small portion of myel-nated ones are degenerated, then start regenerating within 4 days after treatment. At the same time tubulin transport also recovers to normal.2. Neurotoxicity of , '-iminodipropionitrile:Neurological symptoms develop a few days after an intraperitoneal injection of IDPN with dose of 1.5g/kg of body weight. Under these conditions axonal transport of neurofilament proteins is severely inhibited, without any disturbance of that of tubulin and actin. Neurofilament transportbecomes normal 6 weeks after IDPN administration, thoughneurological abnormalities never show recovery.3. Neurotoxicity of acrylamide:Marked acceleration of all components of slow axonal transport is found in rats after 13 week-administration of acrylamide with dose of 200 ppm in drinking water. This finding is interpreted to be the results of axonal degeneration and regeneration.4. Neurotoxicity of 2,5-hexanedione:2, 5-Hexanedione causes an increase of rate of neurofilament transport, leaving that of tubulin and actin unchanged even in early stage of administration when no symptom appears.5. Neurotoxicity of carbon disulfide:This drug shows a similar effect on neurofilament transport to that of 2, 5-hexanedione.

各种神经毒性化学品被认为根据其主要目标分为几类。其中三个主要群体被选中，并在体内模型系统开发，以研究其生化作用机制。通过分析在这些药物的影响下的慢轴突运输，得到以下结果。秋水仙碱的神经毒性：秋水仙碱完全和专门阻断微管蛋白的转运，而不影响肌动蛋白和神经丝蛋白的转运。将秋水仙碱4g注入7周龄大鼠第5腰脊神经节，几乎所有无髓轴突和一小部分有髓轴突变性，并在治疗后4天内开始再生。同时微管蛋白转运也恢复正常. 1，'-亚氨基二丙腈的神经毒性：以1.5g/kg体重的剂量腹膜内注射IDPN后几天出现神经系统症状。在这些条件下，神经丝蛋白的轴突运输受到严重抑制，而微管蛋白和肌动蛋白的轴突运输没有受到任何干扰。IDPN治疗6周后神经丝转运恢复正常，但神经系统异常从未恢复.丙烯酰胺的神经毒性：大鼠在饮用水中给予200 ppm剂量的丙烯酰胺13周后，发现缓慢轴突运输的所有组分均显著加速。这一发现被解释为轴突变性和再生的结果。2，5-己二酮的神经毒性：2，5-己二酮引起神经丝转运速率增加，即使在给药早期未出现症状时，微管蛋白和肌动蛋白的转运速率也保持不变.二硫化碳的神经毒性：该药物对神经丝转运的作用与2，5-己二酮相似。