Is parathyroid hormone present only in secretory granules?
甲状旁腺激素只存在于分泌颗粒中吗?
基本信息
- 批准号:61570152
- 负责人:
- 金额:$ 1.15万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1986
- 资助国家:日本
- 起止时间:1986 至 1987
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Electron microscopical immunohistochemistry on human and bovine parathyroid tissue also revealed cytoplasmic activities for parathyroid hormone besides thosein secretory grammles. Immunohistochemical analysis was performed by unlabeledantibody enzyme method indirectly with whole IgG as the first and second antiserum. Anti human PTH-guinea Rg antibody (Toyogyozo) was anti-syntlolic (1-34)PTH. peptide. speeific activity was determined by absorption test and final absonption concentration of antigen(synthetic PTH1-34) against the antibody was 11 g/ml. The postive reaction was generally intensitive in normal parathyroid tissues, less in hyperplasia, and weak in adenoma. However, the normal tissue adjacent to adenoma was less intensitive than adenoma cells.Electronmicroscopically secretory gramiles were very few but hyghly positive for PTH immunoreactivity and rough ER, and ribosomes were also positive. In order to corroborate the immunohistochemical presence of PTH in cytoplasm, the protein A-gold method was applied for the parathyroid glands of rats which were perfused by glutaraldehyde or periodate-paraformaldehyde in order to prevent the leakage of PTH during useal method of fixation. The positive grams were observed in the cytoplasmic matrix as well as secretory grammles.
电镜免疫组化对人和牛甲状旁腺组织的电镜免疫组化结果显示,除了分泌腺微粒外,细胞质中还有甲状旁腺激素的活性。采用无标记抗体酶法间接免疫组化分析,以全IgG为第一抗血清和第二抗血清。抗人甲状旁腺激素-豚鼠Rg抗体(toyoyozo)是抗合成型(1-34)甲状旁腺激素。肽。通过吸收试验测定抗原(合成PTH1-34)对抗体的最终吸收浓度为11 g/ml。在正常甲状旁腺组织中,阳性反应一般较强烈,在增生中较少,在腺瘤中较弱。然而,邻近腺瘤的正常组织的密度不如腺瘤细胞。电镜下分泌的颗粒很少,但PTH免疫反应性和粗ER呈高阳性,核糖体也呈阳性。为了证实PTH在细胞质中的免疫组化存在,采用蛋白a金法对戊二醛或高尿酸多聚甲醛灌注的大鼠甲状旁腺进行检测,以防止PTH在固定方法中渗漏。细胞质基质及分泌克均呈阳性。
项目成果
期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kunihiko Goto: "Is parathyroid hormone present only in secretory gramules?" Tohoku Journal of Experimental Biology. (1988)
后藤邦彦:“甲状旁腺激素只存在于分泌颗粒中吗?”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kunihiko Goto: "Cells swell, and generate heat at hunctional expression-rotation model-" Xth International Congress of Pharmacology, Sydney, Australia. P450 (1987)
Kunihiko Goto:“细胞在功能表达旋转模型下膨胀并产生热量”,第十届国际药理学大会,澳大利亚悉尼。
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- 影响因子:0
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{{ truncateString('GOTO Kunihiko', 18)}}的其他基金
Are there differences among characteristics of portions of human mesenteric artery trees?
人类肠系膜动脉树各部分的特征是否存在差异?
- 批准号:
10670153 - 财政年份:1998
- 资助金额:
$ 1.15万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Expression of human IL-2 receptor beta and gamma chains in mucosal immue lymphatic tissue and the immunopathological meaning.
人IL-2受体β链和γ链在粘膜免疫淋巴组织中的表达及其免疫病理学意义
- 批准号:
05670160 - 财政年份:1993
- 资助金额:
$ 1.15万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
How is intracellular calcium ion in parathyroidal tumor cells increased?
甲状旁腺肿瘤细胞胞内钙离子是如何增加的?
- 批准号:
63570139 - 财政年份:1988
- 资助金额:
$ 1.15万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)