How is intracellular calcium ion in parathyroidal tumor cells increased?
甲状旁腺肿瘤细胞胞内钙离子是如何增加的?
基本信息
- 批准号:63570139
- 负责人:
- 金额:$ 1.02万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1988
- 资助国家:日本
- 起止时间:1988 至 1989
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In stimulation-secretion coupling, a stimulation induces the increase of intracellular calcium ions, subsequently following that the secretory granules are secreted by the mechanism of reverse pinocytosis. None hormone controls parathyroid chief cells and then by the increase or decrease of extracellular calcium ions the secretion of parathyroid hormone(PTH) is determined. In the present study, the measurement. of intracellular calcium ion transition and the relationship between calcium transition and secretion of PTH are investigated.The human and calf parathyroid tumor tissues were minced, and treated with 0.05% collagenase, subsequently resulting in preparing free cells. The dispersed parathyroid cells were cultured in the Pulbecco's minimum essential medium containing 10% fetal calf serum on a cover glass in Petri dish. They were treated with the loading of 5 muM Fura-2AM for 2 hours. After washing, the cover glass with the cultured cell was seton constant-temperature chamber, and perfused by lianks balanced salt solution. By project-type fluorescence phase-contrast microscopy, one cell was projected on emission of 340nm and 380 nm. The emission irradiated by one cell runs through 510nm band pass filter, and was received in ultra- sensitive video-camera. The images of the cell emission on the video-camera was expressed on oscillograph, and was drew on paper by penrecorder. The intracellular calcium ions was gradually increased in number for 5 msec after stimulation by hypocalcium concentration of perfusion fluid and them rapidly reached the highest point within the first 10 msec. The calcium transient was gradually decreased to basal point at 40 msec.
在刺激-分泌偶联中,刺激诱导细胞内钙离子增加,随后通过反向胞饮机制分泌颗粒。没有激素控制甲状旁腺主细胞,然后通过细胞外钙离子的增加或减少来决定甲状旁腺激素(PTH)的分泌。在本研究中,测量。本文研究了人和小牛甲状旁腺肿瘤细胞内钙离子的转移及其与甲状旁腺激素分泌的关系。将分散的甲状旁腺细胞培养在皮氏培养皿中的盖玻片上的含有10%胎牛血清的Pulbecco最低必需培养基中。它们用5 μ M Fura-2AM负载处理2小时。冲洗后,将装有培养细胞的盖玻片置于恒温箱中,用Lianks平衡盐溶液灌注。通过投影型荧光相差显微镜,将一个细胞投影在340 nm和380 nm的发射上。一个细胞发出的辐射穿过510 nm带通滤光片,并被超灵敏摄像机接收。将摄像机拍摄的细胞发射图像用电子照相机表示,并用笔式记录仪记录在纸上。在低钙浓度灌注液刺激后5毫秒内,细胞内钙离子逐渐增多,并在前10毫秒内迅速达到最高点。钙瞬变逐渐降低至40 msec时的基础点。
项目成果
期刊论文数量(23)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
後藤邦彦: "副甲状腺腫瘍細胞の細胞内カルシウム濃度の測定" 日本内内泌学会雑誌.
Kunihiko Goto:“甲状旁腺肿瘤细胞内钙浓度的测量”日本内分泌学会杂志。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kunihiko Goto: "Cells swell,and generate heat at functional expression-rotation model-" Xth Internat.Cong Pharmacol.Sydney. 10. 450 (1987)
Kunihiko Goto:“细胞在功能表达旋转模型下膨胀并产生热量”Xth Internat.Cong Pharmacol.Sydney。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Goto,Kunihiko: "Cells swell,and generate heat at functional expression-rotation model" Xth Internat.Cong.Pharmacol.450 (1987)
Goto, Kunihiko:“细胞在功能表达旋转模型中膨胀并产生热量”Xth Internat.Cong.Pharmacol.450 (1987)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Goto, K.: "Cells swell, and generate hert at functional expression-rotation model" Xth Internat.Cong. Pharmacol.Sydney.p450, 1987.
Goto, K.:“细胞膨胀,并在功能表达旋转模型中产生赫特”Xth Internat.Cong。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Goto, K.: "Transmembrane macromolecule rotation model molecular hypothesis of membrane excitation" Tohoku J. Exp. Med. 139:159-164, 1983.
Goto, K.:“膜激发的跨膜大分子旋转模型分子假说”Tohoku J. Exp。
- DOI:
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- 影响因子:0
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{{ truncateString('GOTO Kunihiko', 18)}}的其他基金
Are there differences among characteristics of portions of human mesenteric artery trees?
人类肠系膜动脉树各部分的特征是否存在差异?
- 批准号:
10670153 - 财政年份:1998
- 资助金额:
$ 1.02万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Expression of human IL-2 receptor beta and gamma chains in mucosal immue lymphatic tissue and the immunopathological meaning.
人IL-2受体β链和γ链在粘膜免疫淋巴组织中的表达及其免疫病理学意义
- 批准号:
05670160 - 财政年份:1993
- 资助金额:
$ 1.02万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Is parathyroid hormone present only in secretory granules?
甲状旁腺激素只存在于分泌颗粒中吗?
- 批准号:
61570152 - 财政年份:1986
- 资助金额:
$ 1.02万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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