Plasmids and pathogenicity of Salmonella braenderup and Salmonella typhimurium

布伦德鲁普沙门氏菌和鼠伤寒沙门氏菌的质粒和致病性

基本信息

批准号：
61570204
负责人：
HORIUCHI Sankichi
金额：
$ 1.34万
依托单位：
Tokyo Medical and Dental University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

Correlation of pathogenicity and plasmids of Salmonella braenderup and Salmonella typhimurium isolated from gastroenteritis patients was examined. Of 40 S. braenderup isolates, 38 strains (95%) carried a plasmid of 74 megadalton (Mdal) in size and 17 (31%) of 55 S. typhimurium isolates carried a 60-Mdal plasmid. Plasmid was eliminated from one of the strains by incubaton in L-broth containing ethidium bromide at 42C.Strains of plasmid-bearing and plasmid-eliminated S. braenderup showed similar pathogenicity for invasiveness to HeLa cell, infectivity to the guinea pig eyes, and fluid accumulation in the rabbit ligated ileal loop. Intravenous inoculation of ddY mice with the parent strain produced 100% mortality with characteristic signs of septicemia, whereas inoculation of the plasimd-eliminated strain produced 0% mortality when 10^8 organisms were inoculated. These results suggested that virulence genes for gastroenteritis except for mice lethality are carried by the chromosome of S. braenderup. However, it appeared that the 74・Mdal plasmid may contribute to virulence in mice lethality. The same tests were performed on four strains of S. typhimrium, two of which carried the 60-Mdal plasmid, one of which had been eliminated for plasmid, and one of which was plasmid-free. It was revealed that the 60-Mdal plasmid was not essential for the pathogenicity of S. typhimurium except for the ability evolving mouse typhoid.Scanning electron micrographic studies of the rabbit ligated ileal loops revealed that both of the plasmid-bearing and plasmid-free strins of S. braenderup and S. typhimurium destructed villi and microvilli.

检查了从胃肠炎患者中分离出的致病性和质粒的致病性和质粒的相关性。在40链球菌分离株中，有38个菌株（95％）的质粒具有74兆达尔顿（MDAL）的质粒，而55孢子虫分离株中有17个（31％）带有60毫米质粒。 Incubaton在42c的含有溴化乙锭的L植物中从其中一种菌株中消除了质粒。质粒和质粒的braenderup的质粒和质粒的s。braenderup均显示出对Hela细胞的侵入性的类似致病性，对豚鼠的感染，对豚鼠的感染，并在兔子ligbit的ligbit ligbit ligbit ligbit ligeal iLigeal ligeal ligbit ligit。静脉内接种母亲菌株的DDY小鼠产生100％死亡率，具有特征性败血病的特征性迹象，而接种10^8生物体时，降低血浆菌株的接种产生了0％的死亡率。这些结果表明，除小鼠杀伤力外，胃炎的病毒基因由S. braenderup的染色体携带。然而，似乎74・MDAL质粒可能导致小鼠致死性病毒。对四个鼠伤寒链芽孢杆菌进行了相同的测试，其中两个携带了60-MDAL质粒，其中一个被消除以用于质粒，其中一个是无质粒的。据揭示，除了能够进化小鼠伤寒的能力外，60个质质粒对于鼠伤寒链球菌的致病性并不是必不可少的。对兔子卵形环的扫描电子显微照片研究揭示了质体含量和无质体和typhimurium S. typhimurium s. typhimurium seltrofi andicrofi and Icrofi and Icrofi and Icrofi and s. braenderup and s.