A Study on the Toxic Effect of Cadmium on Rat Bones: Electron Probe Micro-Analysis

镉对大鼠骨骼毒性作用的研究：电子探针显微分析

• 批准号: 61570264
• 负责人: MORIYAM Tadashige
• 金额: $ 1.09万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570264/
• 关键词: Cadmium; Rat; Bone; EPMA; 骨強度; 骨微細構造; X線マイクロアナリシス; EPMA

The purpose of our study is to prove experimentally the direct effect of cadmium on bones. In our perious study, the mechanical strength of the bones of young rats was clearly decreased and it was suggested that the decrease in the mechanical strength was due to the abnormal change in the microstructure of the bone. In orger to prove this point, EPMA technique was used to analyze the changes in the elements of bones and to examine the cadmium localization in bones.Humeri of young rats, administered with 10ppm of cadmium for 4 weeks, were dissected out from the bodies, fixed in the ethanol at -20゜C, then dried and cut to obtain the clear surface for the EPMA. these samples was gold coated on a carbon plate and analysed by the WDX type-electron probe microanalyzer, EPM-810 (Schimazu Corporation, Japan) in the Faculty of Engineering,Osaka City University.Elements, detected by EPM Analyzer and identified by the charasteristic X ray wavelength, are Ca: K<alph>, K<bet>, P: K<alph>, K< bet>, Na: K<alph>, K: K<alph>, Mg: K<alph>, S: K<alph>, Cl:K<alph>, Zn: K<alpha>, O:K<alph>, F: K<alph> and Fe: K<alph>. Both in the control and Cd groups, Ca, P, Na, K, Mg and Cl are found abundantly on every spot (10<micr>m in diameter).S. Zn, Fe and F are not detected everywhere, but it can be proved that these elements exist in some places in bones. In the bones of Cd group, it is not found any change in the distribution of these elements. Cd is hard to detect in this method. We have detected the small sign of Cd locarization in the epiphysial zone, and not jet found out the precise site of the cadmium localization.

本研究的目的是通过实验证明镉对骨骼的直接影响。在我们的前期研究中，幼鼠骨的机械强度明显下降，这表明，机械强度的下降是由于骨的微观结构的异常变化。为了证明这一点，我们用EPMA技术分析了骨元素的变化和镉在骨中的定位，用10 ppm镉染毒4周的幼鼠，从体内解剖出肱骨，用-20 ℃乙醇固定，然后干燥并切割，以获得EPMA所需的透明表面。这些样品被涂在碳板上，用大坂市立大学工学部的WDX型电子探针微量分析仪（日本Schimazu公司生产）进行分析，用XRD分析仪检测并通过特征X射线波长鉴定的元素为Ca：K<alph>、K<bet>、P：K<alph>、K< bet>、Na：K<alph>、K：K<alph>、Mg：K<alph>、S：K<alph>、Cl：K<alph>、Zn：K<alpha>、O：K<alph>、F：K<alph>和Fe：K<alph>。在对照组和镉组中，Ca、P、Na、K、Mg和Cl在直径为10 m的每个点上都有丰富的分布<micr>。Zn、Fe和F并不是到处都能检测到，但可以证明这些元素存在于骨骼中的某些地方。在Cd组的骨骼中，这些元素的分布没有发现任何变化。镉在该方法中很难检测。我们在骨骺区检测到了镉定位的小征象，并没有找到镉定位的确切位置。

项目成果

Kumiko Ogoshi, Tadashige Moriyama and Yukuo Nanzai: "A Study on the Toxic Effect of Cadmium on Rat Vones: Electron Probe Micro-Analysis" 4th IUPAC Cadmium Workshop. 9. (1988)

Kumiko Ogoshi、Tadashige Moriyama 和 Yukuo Nanzai：“镉对大鼠 Vones 毒性作用的研究：电子探针微分析”第四届 IUPAC 镉研讨会。