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Investigation of tramsporting property of amino acid and glucose in the rat intestinal epithelial ER.

大鼠肠上皮ER中氨基酸和葡萄糖转运特性的研究。

基本信息

批准号：
61570335
负责人：
KANEKO Eizo
金额：
$ 1.41万
依托单位：
Hamamatsu University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

The endoplasmic reticulum (ER) -enriched microsomes were obtained from rat intestinal mucosal cells by a subfractionation technique to investigate transporting property of amino acids and glucose. Nacl gradient had no effect on the time course of L-alanine and D-glucose uptakes, and the data suggested that the response to medium osmolarity chamges of these microsomes reflected diffusion or binding to the membrane of thes substrates. also, D-glucose binded to the memlrane than L-alanine The specific activities of G-6-Pase in intestinal ER of mouse and guinea pig were ten-fold higher than that of rat , in which the specific activity of G-6-Pase of ER was not significantly different from the homogenate But Acyl-CoA:monoglyceride acyltransferase activity in the not ERwas five-fold higher than that of homogenate. These results suggeoted that ER of the rat inteotinal epithel:um is not important in transport of amino acid and glucose and that Acyl-GoA:monoglyceride acyltransferase is a satisfactory morker enzyme for ER of the rat small intestine, but G-6-Pase not.

采用亚分级分离技术从大鼠肠粘膜细胞中分离出富含内质网的微粒体，以研究其转运氨基酸和葡萄糖的特性。NaCl梯度对L-丙氨酸和D-葡萄糖摄取的时间过程没有影响，数据表明这些微粒体对介质渗透压变化的反应反映了这些底物的扩散或结合到膜上。D-葡萄糖比L-丙氨酸更易与膜结合。小鼠和豚鼠小肠ER中G-6-Glucose的比活性比大鼠高10倍，其中G-6-Glucose的比活性与匀浆无显著差异，而非ER中酰基辅酶A：单甘酯酰基转移酶的活性比匀浆高5倍。提示大鼠小肠上皮ER对氨基酸和葡萄糖的转运不起重要作用，酰基-GoA：单甘酯酰基转移酶是大鼠小肠ER的理想的标记酶，而G-6-葡萄糖酰基转移酶则不是。