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19F-NMR studies on brain metabolism

脑代谢的 19F-NMR 研究

基本信息

批准号：
61570383
负责人：
YUASA Tatsuhiko
金额：
$ 1.47万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570383/
关键词：
NMR spectroscopy Brain edema 31P 19F 脳虚血

项目摘要

We have investigated various metabolic changes occuring during brain ishemia by using NMR spectroscopy. A brain ischemic model adoped here was a 30 min ischemia caused by ligation of bilateral caroted arteries of gerbil mice. A coupled of NMR sudies were carried on this model,which included 1-H NMR,31-P NMR,23-Na NMR, and 19-F NMR.Immedeately after acute brain ischemia high energy phosphate compounds like phosphocreatin, and ATP measured by 31-P NMR spectrometer decreased promptly, and inorganicphosphate built up. But these changes in high energy phosphate compounds returned to the initial state within 30 min after reperfusion of the circulation. The volume of NMR sensitive sodium was decreased just after initiation of the brain ischemia,which meant deterioration of Na pump of cell membran occured. Although the energic state returned to normal level after reperfusion of the blood,brain edema continuously progressed which could be detected by using Gd-DTPA enhanced H NMR imaging and T-1 relaxometry on the ischemic gebil brain.The functional state of blood brain barrier could be monitored when we appleid some NMR sensitive substrate to the ischemic model animals. 5-fluorouracil is one of challengeable substrate because of its character of 19F NMR sensitivity and selective permiability of BBB. After 30 min brain ischemia we found that the 19-F NMR signal intensity of 5-FU from the ishemic brain was 2-3 times greater than that of normal control brain. We could detect the deterioration of selectivity of permiability of the BBB after acute brain ischemia by using 19-F NMR spectroscopy.

我们利用核磁共振光谱研究了脑缺血期间发生的各种代谢变化。采用沙土鼠双侧颈总动脉结扎造成30分钟脑缺血模型。对该模型进行了~（1H）NMR、~（31）P NMR、~（23）Na NMR和~（19）F NMR的联用研究，结果表明，急性脑缺血后即刻，磷酸肌酸等高能磷酸化合物和~（31）P NMR测定的ATP含量迅速下降，无机磷酸盐含量增加。但这些高能磷酸化合物的变化在循环再灌注后30分钟内恢复到初始状态。脑缺血后即刻，细胞膜钠泵功能下降，对核磁共振敏感的钠离子体积减少。缺血再灌注后，虽然能量状态恢复正常，但脑水肿仍在继续发展，用Gd-DTPA增强的1H NMR成像和T-1弛豫法可检测到脑水肿的进展，应用某些NMR敏感底物可监测血脑屏障的功能状态。5-氟尿嘧啶具有19 F-NMR敏感性和血脑屏障选择性通透性的特点，是一种具有挑战性的底物。脑缺血30分钟后，我们发现缺血脑的5-FU的19-FNMR信号强度比正常对照脑的高2-3倍。我们可以用19-F NMR波谱检测急性脑缺血后血脑屏障通透性选择性的恶化。