Diagnostic, molecular epidemiologic and immunological studies of diarrheal viruses

对轮状病毒和腺病毒的诊断方法、基因组核酸电泳分析和血清型分析进行了发展和比较。还对宿主方进行了免疫学研究。获得了以下结果。(1)胶乳凝集试验，酶免疫试验和其他的发展和比较进行，并在两种病毒的每种方法进行了评价。(2)分子和血清流行病学研究在日本的几个地方进行了几年。发现C组轮状病毒。腺病毒，特别是血清型40和41确定的中和和聚丙烯酰胺凝胶电泳的基因组dna使用限制性内切酶。(3)对健康儿童、胃肠炎患者和其他疾病患者进行抗轮状病毒和腺病毒抗体检测。血清及脑脊液检查怀疑轮状病毒脑炎。本文报告了川崎患者发生轮状病毒和腺病毒医院感染的情况。本文讨论了轮状病毒和腺病毒在原发性免疫缺陷病例中的感染方式和病毒学特征，为轮状病毒感染的诊断和流行病学的临床研究提供了基础。我们计划在下一个实验中研究疫苗和详细分析病毒基因和多肽。

Developments and comparisons of diagnostic methods, electrophoretic analyses of genomic nucleic acids and serotypic analyses were conducted to rotaviruses and adenoviruses. Immunological studies on host sides were also conducted. The following results were obtained.(1) Developments and comparisons of latex agglutination tests, enzyme immunoassy and others were conducted,and each method is evaluated in both viruses.(2) Molecular- and sero- epidemiological studies were conduc ed for several years at several places in Japan. Group C rotavirus was discovered. Adenoviruses, especially serotypes 40 and 41 were determined by neutralization and polyacrylamide gel electrophoresis of genome dnas using restriction enzymes.(3) Anti-rotavirus and anti-adenovirus antibodies were examined in healthy children,in patients with gastroenteritis and in patients with other diseases. Rotavirus encephalitis were suspected from the examinations of serum and cerebrospinal fluid. Nosocomial infection of rotavirus and adenovirus was reported in patients with kawasaki disease. The mode of rotavirus and adenovirus infection and virological feature were discussed in cases of primary immunodeficiencies.These studies contributed to the fundamental clinical researches on diagnosis and epidemiology of infection caused by diarrheal viruses. We are planning to study of vaccine and detail analyses of viral genes and polypeptides at the next experiments.