Study of alloantigen specific human suppressor T cell clone and its clinical application

同种异体抗原特异性人抑制性T细胞克隆的研究及其临床应用

• 批准号: 61570612
• 负责人: FUKUDA Yasuhiko
• 金额: $ 1.54万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570612/
• 关键词: Mixed lymphocyte culture; Human suppressor T cell clone; 非特異的免疫抑制因子; 抑制性T細胞; 同種抗原特異的抑制性T細胞; 抑制性T細胞クローン; サプレッサーT細胞; サプレッサーT細胞のクローニング

We established 72 T cell clones generated from human one way MLC by recombinant IL-2 and feeder cells. Only one clone, -1-C5, had suppressor T cell like fuction, that is this clone showed ability to suppress the MLK but lacked cytotoxic property.-1-C5 clone was not restricted by MHC(HLA-A,B,DR,DG). Its surface marker was CD3 and CD8 psitive.The mechanism od suppression by this clone was due to soluble factor. The soluble factor was produced from this clone under the influence of gamma irradiated original MLR. Interestigly this factor showed non specific suppression. Like this soluble factor may be useful in the field of organ transplantation clinically in the future.

我们用重组IL-2和饲养细胞从人单向MLC中建立了72个T细胞克隆。只有一个克隆，-1-C5，具有抑制性T细胞样功能，即该克隆显示出抑制MLK的能力，但缺乏细胞毒性性质。1-C5克隆不受MHC（HLA-A、B、DR、DG）的限制。其表面标志为CD_3和CD_8阳性，其抑制机制为可溶性因子。在γ射线照射的原始MLR的影响下，从该克隆产生可溶性因子。有趣的是，该因子显示非特异性抑制。这种可溶性因子可能在将来的临床器官移植领域中有用。

项目成果

YAHATA H.;FUKUDA Y.;ASAHARA T.;ONO E.;DOHI K.: Japan J.Exp.Med.56. 257-264 (1986)

YAHATA H.；FUKUDA Y.；ASAHARA T.；ONO E.；DOHI K.：日本 J.Exp.Med.56。

八幡浩,福田康彦,浅原利正,石川哲大,土肥雪彦: 移植. 23. 138 (1988)

Hiroshi Yawata、Yasuhiko Fukuda、Toshimasa Asahara、Tetsuhiro Ishikawa、Yukihiko Doi：移植。 23. 138 (1988)

YAHATA H.;FUKUDA Y.;ISHIKAWA T.;ASAHARA T.;DOHI K.: Transplantation Proceedings. (1989)

YAHATA H.；FUKUDA Y.；ISHIKAWA T.；ASAHARA T.；DOHI K.：移植程序。

YAHATA H.;FUKUDA Y.;TANAKA I.;ASAHARA T.;ONO E.;OMOTEHARA T.;DOHI K.: Transplantation Proceedings. 19. 540-543 (1987)

YAHATA H.；FUKUDA Y.；TANAKA I.；ASAHARA T.；ONO E.；OMOTEHARA T.；DOHI K.：移植程序。

YAHATA H.; FUKUDA Y.; ASAHARA T.; ONO E.; DOHI K.: "Correlation between stable E-rosette formation and induction of alloantigen specific MLR suppressor cells in human mixed lymphocyte culture" Japan J. Exp. Med.56. 257-264 (1986)

八幡H.；