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Study of heterogeneity of human monocytes in transplantation immunology

人单核细胞移植免疫学异质性研究

基本信息

批准号：
01570714
负责人：
FUKUDA Yasuhiko
金额：
$ 1.47万
依托单位：
Hiroshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570714/
关键词：
Transplantation HLA antigen HLA-DQ antigen on Monocyte Monitoring of Rejection Activated Monocyte 免疫学的モニタリング HLAーDQ陽性単球 HLAーDQ抗原 HLA抗原 DQ抗原腎臓移植リンパ球混合培養

项目摘要

The important role of monocytes in immunological reaction has been made clear recently. This study was focused on the change of expression of HLA class 2 antigens(DR, DQ)on the surface of monocytes in transplantation immunology.(Methods)Detections of HLA antigens of monocytes were done by two color flowcytometory.1) As control study, peripheral monocytes from healthy persons, haemodialysis patients and long surviving kidney transplant recipients were examined.2) As in vitro model, monocytes in mixed leucocyte culture were examined.3) As clinical trial, monocytes from kidney transplant recipients were examined serialy before and after surgery.(Results)1)Human monocytes were classified to two groups of DQ positive monocyte (DQ^+Mo) and DQ negative monocyte (DQ^-Mo). In healthy persons 10 to 20% of monocytes had DQ antigen together with DR antigen. Interestingly this DQ^+MO ratio increased dramatically, after three days in mixed leucocyte culture.2)Inkidney, transplant recipients, longsurvival cases showed very low DQ^+Mo ratio but patients suffered from acute rejection showed high DQ^+Mo clearly(mean 52.9%). After the crosis had gone DQ^+Mo returned to normal range very quickly.From our study it became clear that DQ antigen was a sensitive marker of activated human monocytys.Clinically DQ^+Mo in kidney transplant recipients was reliable monitoring test for acute rejection, especially under the use of cyclosporine.

单核细胞在免疫反应中的重要作用近年来已被阐明。本研究旨在探讨移植免疫学中单核细胞表面HLA 2类抗原（DR、DQ）表达的变化。（方法）采用双色流式细胞术检测单核细胞HLA抗原; 1）检测正常人、血液透析患者和长期存活肾移植受者外周血单核细胞作为对照; 2）检测混合白细胞培养物中单核细胞作为体外模型; 3）检测肾移植受者手术前后单核细胞作为临床试验。（结果）1）人单核细胞分为DQ阳性单核细胞（DQ^+Mo）和DQ阴性单核细胞（DQ^-Mo）两组。在健康人中，10%至20%的单核细胞具有DQ抗原和DR抗原。有趣的是，在混合白细胞培养3天后，DQ^+Mo比值显著增加。2）在肾移植受者中，长期存活的患者DQ^+Mo比值很低，而急性排斥患者DQ^+ Mo比值明显升高（平均52.9%）。结果表明，DQ^+ Mo是一种敏感的单核细胞活化标志物，在肾移植受者中，DQ^+Mo是监测急性排斥反应的可靠指标，尤其是在使用环孢素的情况下。