Study of heterogeneity of human monocytes in transplantation immunology

单核细胞在免疫反应中的重要作用近年来已被阐明。本研究主要探讨移植免疫过程中单核细胞表面HLA 2类抗原（DR， DQ）表达的变化。（方法）采用双色流式细胞术检测单核细胞HLA抗原。1)对照研究，检测健康人、血液透析患者和长期存活肾移植受者外周血单核细胞。2)采用体外模型，检测混合白细胞培养中的单核细胞。3)作为临床试验，在手术前后对肾移植受者的单核细胞进行连续检查。（结果）1)人单核细胞分为DQ阳性单核细胞（DQ^+Mo）和DQ阴性单核细胞（DQ^-Mo）两组。在健康人群中，10 - 20%的单核细胞含有DQ抗原和DR抗原。有趣的是，在混合白细胞培养三天后，DQ^+MO比值显著增加。2)在肾移植受者中，长生存期患者DQ^+Mo较低，急性排斥反应患者DQ^+Mo明显较高（平均52.9%）。当交叉消失后，DQ^+Mo很快恢复到正常范围。从我们的研究中可以清楚地看出，DQ抗原是活化的人单核细胞的敏感标记。肾移植受者临床上DQ^+Mo是急性排斥反应的可靠监测指标，特别是在使用环孢素的情况下。

The important role of monocytes in immunological reaction has been made clear recently. This study was focused on the change of expression of HLA class 2 antigens(DR, DQ)on the surface of monocytes in transplantation immunology.(Methods)Detections of HLA antigens of monocytes were done by two color flowcytometory.1) As control study, peripheral monocytes from healthy persons, haemodialysis patients and long surviving kidney transplant recipients were examined.2) As in vitro model, monocytes in mixed leucocyte culture were examined.3) As clinical trial, monocytes from kidney transplant recipients were examined serialy before and after surgery.(Results)1)Human monocytes were classified to two groups of DQ positive monocyte (DQ^+Mo) and DQ negative monocyte (DQ^-Mo). In healthy persons 10 to 20% of monocytes had DQ antigen together with DR antigen. Interestingly this DQ^+MO ratio increased dramatically, after three days in mixed leucocyte culture.2)Inkidney, transplant recipients, longsurvival cases showed very low DQ^+Mo ratio but patients suffered from acute rejection showed high DQ^+Mo clearly(mean 52.9%). After the crosis had gone DQ^+Mo returned to normal range very quickly.From our study it became clear that DQ antigen was a sensitive marker of activated human monocytys.Clinically DQ^+Mo in kidney transplant recipients was reliable monitoring test for acute rejection, especially under the use of cyclosporine.