EXPERIMENTAL RESEARCH FOR FETAL SURGERY

胎儿手术的实验研究

• 批准号: 61570617
• 负责人: AKIYAMA Hiroshi
• 金额: $ 1.54万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570617/
• 关键词: FETAL SURGERY; EXPERIMENTAL FETAL SURGERY; 先天性横隔膜ヘルニア; 横隔膜ヘルニア

This research was designed to creat hypoplastic lungs on fetal rabbits of 20 to 25 days' gestation with surgically producted diaphragmatic henia and to examine possibility of surgical therapy in fetal period. Surgical techniques: General anesthesia was induced with 2.5% sodium thiopenthal and maintained with 50:50 nitrous oxide and oxygen supplemented by 1.0 to 2.0% halothane. The abdomen of the mother rabbit was opened and the uterine horn was delivered out of the abdomen. The left foreleg and left chest wall of one of the fetuses were exposed through the uterotomy. Left diaphragm was ruptured by penetration of a fine hemostat through the chest wall. The operated fetuses were delivered by cesarean section 4 to 5 days after operation. Each fetus was weighed and sacrificed. Both lungs of the fetuses were removed and fixed in 10% neutral formalin solution. The hypoplastic lungs, which histological findings were not so severe, were recognized in fetal rabbits with successfully producted diaphragmatic hernia. To create more severe hypoplastic lung, this procedure should be carried out in yonger fetal rabbits. However, it was very difficult to do this procedure on fetal rabbits before 20 days' gestation. On the other hand, creation of this model on fetal goats has been tried since 1988 because mother goats died of pulmonary complication 2 to 3 days after surgery. To complete this kind of chronic preparation in goats, it will be very important to kaiketu some problems in management of mother goats.

本研究用手术造成的胎兔先天性肺气肿，在胎龄20 ~ 25天的胎兔上制作肺发育不良模型，探讨胎期手术治疗的可能性。手术技术：用2.5%硫喷妥钠诱导全身麻醉，并用50：50的氧化亚氮和氧气补充1.0 - 2.0%氟烷维持。打开母兔的腹部，将子宫角从腹部取出。通过子宫切开术暴露其中一只胎仔的左前腿和左胸壁。细止血钳穿透胸壁导致左横膈膜破裂。术后4 ~ 5天剖宫产分娩。对每只胎仔称重并处死。取出胎仔的双肺并固定在10%中性福尔马林溶液中。在成功制造疝的胎兔中发现了组织学表现不严重的发育不全肺。为了建立更严重的肺发育不良，本手术应在更年轻的胎兔上进行。然而，在妊娠20天之前的胎兔上进行该操作是非常困难的。另一方面，自1988年以来一直尝试在胎羊上建立这种模型，因为母山羊在手术后2至3天死于肺部并发症。为了完成这种慢性制剂在山羊，这将是非常重要的凯克图一些问题，在母山羊的管理。

项目成果

秋山洋, 高松民夫, 野口啓幸, 田原博幸: 日本小児外科学会誌.

Hiroshi Akiyama、Tamio Takamatsu、Hiroyuki Noguchi、Hiroyuki Tahara：日本小儿外科学会杂志。