Fetal surgery:Experimental study of congenital urinary trinary tract obstruction.

胎儿手术：先天性尿路三路梗阻的实验研究。

• 批准号: 61570774
• 负责人: SHIMADA Kenji
• 金额: $ 1.41万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570774/
• 关键词: Fetal surgery; Congenital urinary tract obstruction; Histopathology; 糸状体嚢胞; 胎児手術; 実験モデル

In an attempt to make the experimental models of congenital urinary tract obstructions,we performed the surgery on the fetal rabbit.Twenty-seven pregnant rabbits were operated on between 22 to 24 days of gestation.Surgical intervention was made on 52 fetal rabbits:urethral ligation on 14,urethral and urachal ligation on 20,ureteral ligation on 10, bladder neck ligation on 3,and an incision of fetal skin without manipulation of the urinary tract(sham operation)on 5.Dilatation of the urinary tract was evident in 55%(26/47)of the animals. All of the operated newborns were markedly underweight in compared with the unoperated controls. Significant decrease of lung wight/body weight was evident in rabbits with successful urethral ligation.Histologic section of the kidney from ureteral ligation revealed marked parenchymal atrophy. There were small glamerular cysts in the subcapsular zone,as well as severe dilatation of tubules and ducts. The ureter and renal pelvis were dilated above the obstruction in animals with uerteral ligation. The epithelial layer was overstreched,and there was sligh increase in the amount of collagen fibers in the submucosal layers.

In an attempt to make the experimental models of congenital urinary tract obstructions,we performed the surgery on the fetal rabbit.Twenty-seven pregnant rabbits were operated on between 22 to 24 days of gestation.Surgical intervention was made on 52 fetal rabbits:urethral ligation on 14,urethral and urachal ligation on 20,ureteral ligation on 10, bladder neck ligation on 3,and an incision of fetal在55％（26/47）的动物中，没有对尿路进行尿路（假手术）的皮肤。与无手术对照相比，所有手术的新生儿都显着减少了体重。在成功的尿道结扎术中，兔子的肺怀特/体重显着降低。输尿管连接的肾脏学部分显示出明显的实质萎缩。在亚囊囊区域中有小的魅力囊肿，以及小管和管道的严重扩张。用uerteral结扎的动物在障碍物上方扩张输尿管和肾脏骨盆。上皮层被过度处理，粘膜下层的胶原蛋白纤维量的增加幅度很高。

项目成果

Kenji Shimada: "Microsurgery and fetal surgery in pediatric urology" Jpn.J.Pediatr.Surg.19. 1185-1191 (1987)

Kenji Shimada：“小儿泌尿外科显微外科和胎儿外科”Jpn.J.Pediatr.Surg.19。

島田憲次: 小児外科. 19. 1185-1191 (1987)

Kenji Shimada：小儿外科。19. 1185-1191 (1987)

Kenji Shimada,Keizou Taguchi: "The experimental model of congenital urinary tract obstruction" Jap.J.Urol.

Kenji Shimada、Keizou Taguchi：“先天性尿路梗阻的实验模型”Jap.J.Urol。

島田憲次,田口恵造: 日泌尿会誌.

Kenji Shimada、Keizo Taguchi：日本泌尿学会杂志。