Sister chromatid exchanges in mice resulting from alcohol consumption during pregnancy.

怀孕期间饮酒导致小鼠姐妹染色单体交换。

• 批准号: 61570780
• 负责人: TAKABAYASHI Toshifumi
• 金额: $ 1.41万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570780/
• 关键词: in vivo SCE; mouse; fetus; 胎仔; アルコール; ニコチン; in vivo SCE(Sister chromatid Exchange)

In vivo sister chromatid exchanges (SCEs) was investigated in mice and fetuses after resulting maternal alcohol consumption during pregnancy. 5-bromodeoxyuridine(BrdU 50 g/g/hour) was continuously infused from the mouse tail vein by the pumping device (IVAC 700). Seventeen hours later, the animals were killed, the maternal bone marrow cells and the fetal liver cells treated to exhibit SCEs. In the bone marrow cells, the frequencies of SCEs were 2.80 0.14(mean SE) in control, 3.71 0.35 (alcohol total dose 5.0g/kg), 4.55 0.39(7.5g/kg) and 5.24 0.37(10.0g/kg) in the cells of alcohol ingestion. The number of exchanges in the bone marrow cells was directly proportional to the amount of alcohol. Also the frequency of the fetal SCEs after 25% alcohol consumption on the 12th day of gestation was greater than the control SCEs. Because this result in the induction of SCEs in the bone marrow cells, I feel this subject merits further study.

体内姐妹染色单体互换(SCES)的研究，在小鼠和胎儿后，导致母亲在怀孕期间饮酒。5-溴脱氧尿嘧啶核苷(BrdU 50g/g/h)由小鼠尾静脉持续注入(IVAC700)。17小时后，动物被处死，母亲的骨髓细胞和胎儿的肝细胞被处理以显示SCES。在骨髓细胞中，正常对照组为2.80±0.14(平均SE)，酒精摄入组为3.71±0.35(酒精总剂量5.0g/kg)，4.55±0.39(7.5g/kg)，5.24±0.37(10.0g/kg)。骨髓细胞的交换次数与饮酒量成正比。孕12天饮酒25%后胎儿SCES发生率明显高于对照组。因为这会导致在骨髓细胞中诱导SCES，所以我觉得这个课题值得进一步研究。