The similarity in drug receptore in maxillary arterial smooth muscle of rabbit between those in anterior byssus retractor muscle of mytilus

家兔上颌动脉平滑肌与贻贝前足丝牵开肌药物受体的相似性

• 批准号: 61570896
• 负责人: HAJIME Mutakami
• 金额: $ 1.22万
• 依托单位: Nihon University, School of Dentistry at Matsudo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570896/
• 关键词: Maxillary arterial smooth muscle; Molluscan smooth muscle; Potentiating action of contraction; 5-HT1a agonists; 5-HT1b agonists; FMRFamide; Cyclic AMP; FMRFamide; serotonin受容体; dopamine受容体; serotonin受容体作用薬; dopamine受容体作用薬

Firstly, the present study was to investigate the similarity in the receptors potentiating the druginduced contraction in the maxi-lary aterail smooth muscle of rabbir (MASM) and those in the anterior byssus retractor muscle of Mytilus (ABRM). Secondly, the receptors mediating the contractile response to serotomin (5-HT) in the MASM were compared those mediating the relaxant response to 5-HT in the ABRM using some 5-HT1A and 5-HT1B agonists. 1. The norepinephrine (NE)- and histamine (His) induced contractions in the MASM and ACh-induced contraction in the ABRM were potentiated by 5methoxytryptamine (5-MeOT) and 5-methoxy-N,N-dimethyltryptamine (5-MeODMT), but not by 1-(3-cholorophenyl) piperazien (mCPP), 8-hydroxy-dipropylaminotetraline (8-OH-DPAT), m-trifluromethylpiperazxine (TEMPP), p-aminophenylethyl-TEMPP (PAPP), 1-(2-methoxyphenyl)piperazien (2MPP) and quipazine. The potentiating actions of NE-, His- and ACh-contractions by 5-MeOT and 5-MeODMT were not prevented by 5-HT antragoni … More sts, ketanserine, 1-(1-naphthyl)piperazine (NAP), mianserine, cyproheptadine and methysergide. The potentiating actions of the contracions by FMRFamide were not prevented by cholecystokinin (CCK) antagonists, benzotript and proglumide, and also by CCK itseld, CCK-4 and CCK-8. 2. The MASM was contracted by 5-MeOT, 5-MeODMT, 8-OH-DPAT, mCPP and quipazine, but not by TEMP P, PAPP and 2MPP, wihle the ABRM was relaxed by 5-MeOT, 5-MeODMT, mCPP, 8-OH-DPAT, TFMPP and PAPP, but not by 2MPP and quipazine. Furthermore, the cyclie AMP (cAMP) levels in the ABRM were elevated by 5-HT, and decreased by 5-MeOT, 5-MeODMT, TFMPP, 2MPP and quipazine, but were not chaged by PAPP and mCPP. Additionally, The ACh-contraction in the ABRM was reversibly inhibited by benextramine and dibenamine, but not by prazocine and yohinbine. The ABRM was relaxed by SCH23390 and the SCH23390-induced relaxation was antagonized by NAP competitibely.From the above experimental results, there are some similatities between both the muscles in their pharmacological properties, that is to say, the drug-induced contractions are potenited by the indole 5-HT_<1a> agonists and FMRFamide, and 5-HT receptors in both MASM and ABRM are predominantly 5-HT1a subtype. However, the changes in cAMP levels in the ABRM induced by 5-HT1a and 5-HT1b agonists do not seen to be linked to the relaxation induced by the agonists. Moreover, There is possibility that 5-HT-_2like receptors are present in the ABRM. Benextramine and dibenamine do not form the covalent sttacvhment to the ACh receptors in the ABRM. Less

本研究首先探讨了增强家兔最大心房肌（MASM）和贻贝前足丝缩肌（ABRM）药物性收缩的受体的相似性。其次，用5-HT_（1A）和5-HT_（1B）激动剂比较了MASM中介导5-HT收缩反应的受体和ABRM中介导5-HT舒张反应的受体。1. 5-甲氧基色胺（5-MeOT）和5-甲氧基-N，N-二甲基色胺可增强去甲肾上腺素（NE）和组胺（His）引起的MASM收缩和ACh引起的ABRM收缩（5-MeODMT），但不是由1-（3-氯苯基）哌嗪（mCPP），8-羟基-二丙氨基四氢化萘（8-OH-DPAT）、间三氟甲基哌嗪（TEMPP）、对氨基苯乙基-TEMPP（PAPP）、1-（2-甲氧基苯基）哌嗪（2 MPP）和喹嗪。5-HT安宫素不能阻断5-MeOT和5-MeODMT对NE、His和ACh收缩的增强作用 ...更多信息 sts、酮丝氨酸、1-（1-萘基）哌嗪（NAP）、米安色林、赛庚啶和麦角新碱。缩胆囊素（CCK）拮抗剂苯并曲普（benzotript）和丙谷胺（proglumide）以及CCK-4和CCK-8均不能阻止FMRFamide的增强收缩作用。2. 5-MeOT、5-MeODMT、8-OH-DPAT、mCPP和quipazine收缩MASM，而TEMP、PAPP和2 MPP不收缩MASM; 5-MeOT、5-MeODMT、mCPP、8-OH-DPAT、TFMPP和PAPP舒张ABRM，而2 MPP和quipazine不舒张ABRM。5-HT使ABRM中环腺苷酸（cAMP）含量升高，5-MeOT、5-MeODMT、TFMPP、2 MPP和喹嗪使cAMP含量降低，而PAPP和mCPP对cAMP含量无影响。此外，苯甲曲明和二苯甲胺可可逆地抑制ABRM的ACh收缩，而哌唑嗪和育亨宾则不可逆地抑制ACh收缩。结果表明，两者在药理学性质上有一定的相似性，即吲哚类5-HT<1a>激动剂和FMRFamide均能增强药物引起的收缩作用，MASM和ABRM的5-HT受体均以5-HT 1a亚型为主。然而，5-HT 1a和5-HT 1b激动剂诱导的ABRM中cAMP水平的变化与激动剂诱导的舒张无关。ABRM内可能存在5-HT-2样受体。苯甲曲明和二苯甲胺不与ABRM中的ACh受体形成共价结合。少

项目成果

Toshinori Ishikawa and Hajime Murakami: "Potentiation of acetylcholine-induced contraction by indole containing 5-HT_<1a> agonists in molluscan smooth muscle of Mytilus"

Toshinori Ishikawa 和 Hajime Murakami：“含有 5-HT_<1a> 激动剂的吲哚对贻贝软体动物平滑肌中乙酰胆碱诱导的收缩的增强作用”

Hajime Murakami;Masakazu Sano;Takashi Tsukimura and Akira Yamazaki: Comparative Biochemistry and Physiology Part C. 98C. 1-7 (1988)

Hajime Murakami；Masakazu Sano；Takashi Tsukimura 和 Akira Yamazaki：比较生物化学和生理学第 C. 98C 部分。

Hajime Murakami, Masakazu Sano, Takashi Tsukimura and Akira Yamazaki: "The relaxation induced by indole and nonindole 5-HT agonists in the molluscan smooth muscle" Comp. Biochem. Physiol.98C. 1-7 (1988)

Hajime Murakami、Masakazu Sano、Takashi Tsukimura 和 Akira Yamazaki：“吲哚和非吲哚 5-HT 激动剂在软体动物平滑肌中诱导的松弛”比较。

Hajime Matukami: "Potentiation of norepinephrine- and histamine-induced contraction by FMRFamide in rabbit maxillary arterial smooth muscle"

Hajime Matukami：“FMRFamide 增强兔上颌动脉平滑肌去甲肾上腺素和组胺诱导的收缩”