Involvement of Protein Phosphorylation in the Degranulation from Rat Basophilic Leukemia Cells

蛋白质磷酸化参与大鼠嗜碱性白血病细胞脱颗粒

• 批准号: 61571081
• 负责人: TERAO Tadao
• 金额: $ 1.41万
• 依托单位: National Institute of Hygienic Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61571081/
• 关键词: Rat; Basophilic Leukemia cell; RBL-2H3; Deqranulation; IqE Receptor; Myosin Light Chin; Protein Phosphorylation; Protein Kinase C; Cytochalasins; Neomycin; K252a; スタウロスポリン; lgEレセプター; RBL2H3

In this study,we inverstigate the inhibitory mechanism of various cytochalasins on the serotonin release from RBL-2H3 cells. All cytochalasins which inbibit the serotonin release suppressed the protein kinase C activity and the phosphorylation of 36 kDa membrenous protein. On the other hand, cytochalasins which engance the release did not inhibit the protein kinase C and the phosphorylation of 36 kDa protein.Enhancement of the phosphorylation of 18 kDa protein was also observed by the antigen (DNP-BSA) stimulation. The phosphorylation of this protein was Ca^<2+>-dependent. The phosphorylated protein was precipitated with actomyosin and behave as a myosin light chain.we,furthermore,studied the effect of various drugs on the degranulation from RBL-2H3 cells. phosphoinosiitdes (PI) turnover and diacylglycerol (DG) formation were enganced by the antigen stimulation. However,when the formation of DG was inhibited by neomycin,the serotonin release was suppressed in proportion to the degree of the inhibition of DG formation. All of these results support our concept that the phosphorylation of 36 kDaprotein by protein kinase C is essential for the degranulatuion from RBL-2H3 cells

在本研究中，我们探讨了不同细胞松弛素抑制RBL-2H3细胞释放5-羟色胺的机制。抑制5-羟色胺释放的细胞松弛素能抑制蛋白激酶C的活性和36 kDa膜蛋白的磷酸化。另一方面，促进释放的细胞松弛素并不抑制蛋白激酶C和36 kDa蛋白的磷酸化。18 kDa蛋白的磷酸化也被抗原(DNP-BSA)刺激而增强。该蛋白的磷酸化依赖于钙离子。用肌动球蛋白沉淀磷酸化蛋白，形成肌球蛋白轻链。我们进一步研究了不同药物对RBL-2H3细胞脱颗粒的影响。抗原刺激促进了磷脂酰肌醇(PI)的周转和二酰甘油(DG)的形成。然而，当新霉素抑制DG的形成时，5-羟色胺的释放与DG形成的抑制程度成正比。所有这些结果都支持我们的观点，即蛋白激酶C对36kDa蛋白的磷酸化是RBL-2H3细胞脱颗粒所必需的

项目成果

R.Teshima, K.Suzuki, H.Ikebuchi, T.Terao: "Engancement of the Phopshorylation of Membrane-Bound Myosin Light Chain by Antigen Stimulation in Rat Basophilic Leukemia Cells"

R.Teshima、K.Suzuki、H.Ikebuchi、T.Terao：“大鼠嗜碱性白血病细胞中抗原刺激对膜结合肌球蛋白轻链磷酸化的影响”