Studies on the Regulation of Immune Functions by Growth Hormone.

生长激素调节免疫功能的研究。

• 批准号: 02671027
• 负责人: TERAO Tadao
• 金额: $ 1.34万
• 依托单位: National Institute of Hygienic Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02671027/
• 关键词: Growth hormone; Growth hormone receptor; IM-9 cells; Monoclonal antibodies; Ubiquitin; Down-regulation; Phorbol diesters; Phosphorylation; IMー9; 抗成長ホルモン受容体抗体; ハイブリド-マ; エビキチン; 抗ユビキチン抗体

Growth hormone-dependent proliferation of IM-9 cells under low-serum conditions was inhibited by the addition of phorbol diesters. The phorbol diesters were found to down-regulate the growthhormone receptor levels. These regulations by phorbol diesters were inhibited by staurosporine or sphingosine, suggesting the involvement of C-kinases. It was also found that phosphorylation of a 55 kDa protein, which was associated with HGHR, was increased.Monoclonal anti-peptide antibodies against human growth hormone receptor(HGHR)were prepared and characterized. Among them, the monoclonal antibody GHRP2-88 was reactive with the extracellular domain of HGHR and the most efficient when used for immunoblotting of IM-9 cell membrane proteins.Monoclonal antibodies against ubiquitin were prepared for the purpose of elucidating the role of hGHR-bound ubiquitin.

佛波酯可抑制低血清条件下IM-9细胞生长激素依赖性的增殖。佛波醇双酯被发现下调生长激素受体水平。佛波二酯的这些调节被星形孢子素或鞘氨醇抑制，这表明C-激酶参与其中。此外，还发现与HGHR相关的55 kDa蛋白的磷酸化程度增加。制备并鉴定了抗人生长激素受体(HGHR)的单抗。其中，单抗GHRP2-88与人生长激素受体胞外区发生反应，是免疫印迹IM-9细胞膜蛋白最有效的抗体。

项目成果

Y.Saito,et al.: "Production and Characterization of Monoclonal Anti-Peptide Antibodies Specific for Human Growth Hormone Receptor."

Y.Saito 等人：“人类生长激素受体特异性单克隆抗肽抗体的生产和表征”。