Removal of Fatty Soil by Lipase - Detergent systems

测定了脂肪酶的相对活性、黄OB的增溶作用和三油苷的去除作用，以阐明脂肪酶水解和增溶作用对脂肪酶-表面活性剂体系中三油苷去除的影响。本实验采用了三种脂酶，分别来自圆柱假丝酵母、镰刀菌和粘胶色杆菌。在脂肪酶- SDS或LAS体系中，随着表面活性剂浓度的增加，脂肪酶的活性逐渐降低。当表面活性剂的浓度较低时，脂肪酶的活性得到很好的维持，并通过水解促进三油苷的去除。另一方面，当表面活性剂的浓度较高时，活性被抑制，三油酸被增溶去除。结果表明，水解法的去除率高于溶解法。在脂肪酶- aos或AE_2S体系中，无论是高浓度还是低浓度的表面活性剂，三油苷都被高度去除。这是由于低cmc的表面活性剂能很好地保持脂肪酶的活性和增溶作用。

The relative activity of lipase, solubilization of Yellow OB and removal of triolein were determined to clarify the effects of hydrolysis by lipase and solubilization on removal of triolein in the lipase-surfactant system. Three kinds of lipases from Candida cylindracea, Fusarium sp. and Chromobacterium viscosum were used.In the lipase- SDS or LAS system, the activity of lipase was gradually reduced as the concentration of the surfactant was increased. When the concentration of the surfactant is low, the activity of lipase was well maintained and the removal of triolein was promoted by hydrolysis. On the other hand, in case that the concentration of the surfactant is high, the activity was inhibited and triolein was removed by solubilization. It was found the removal by hydrolysis was higher than that by solubilization.In the lipase-AOS or AE_2S system, triolein was highly removed in both high concentration and low concentration of the surfactant. This is explained by good maintaining of the activity of lipase and solubilization caused by low cmc of the surfactant.