Studies on the mechanisms of metabolic control in post-embryonic development of insects, emphasizing on the post-transcriptional modifications

在昆虫胚胎发育过程中，其代谢发生了剧烈的变化，酚氧化酶的激活机制与表皮发生和防御机制的关系已被分析。Ashida分析了家蚕血淋巴酚氧化酶原的级联系统，包括几种蛋白酶。Yamazaki研究了新蜕皮角质层中的漆酶型氧化酶。Ohnishi分析了激活后释放酚氧化酶原的片段的结构。Natori研究了肉蝇Sarcophage的防御系统，并分析了2种受损伤诱导的肽的生物合成的控制机制。利用几丁质亲和层析，Tomino分离出几种角质层特异性蛋白质，并研究了这些蛋白质表达的时间模式。奇诺研究了脂肪动力激素对蝗虫脂转运蛋白系统的修饰。Takahashi分离了cGMP依赖性蛋白激酶。Yamashita研究了黄颡鱼卵的特异性蛋白质及其蛋白酶。镜山从鱼卵中分离出一种新型巯基蛋白酶。这些研究揭示了昆虫代谢控制机制的各个阶段，并在分子水平上分析了这些机制。

In post-embryonic development of insects, drastic changes in metabolism could be found. Mechanisms of phenoloxidase activation have been analyzed in relation to cuticulogenesis and to defense mechanism. Ashida analyzed cascade system, involving several proteases, of pro-phenoloxidase of bombyx hemoymph. Yamazaki studied laccase-type oxidase in newly ecdysed cuticles. Ohnishi analyzed structure of the fragment released the prophenoloxidase upon activation. Natori has worked on defense system of the fleshfly, Sarcophage, and analyzed control mechanism of biosynthesis of 2 kinds of peptides, which are inducible by injury. Using affinity chromatography of chitin, Tomino isolated several cuticle-specific proteins and studied temporal pattern of expression of these proteins. Chino studied lipophorin system of locust as modified by adipokinetic hormone. Takahashi isolated cGMP-dependent protein kinase from Bombyx. Yamashita studied egg-specific protein and its protease of Bombyx eggs. Kageyama isolated new type of thiolprotease from Bombyx eggs. These studies revealed various phases of metabolic control mechanisms of insects and the mechanisms were analyzed at a molecular level.