Studies on the mechanisms of metabolic control in post-embryonic development of insects, emphasizing on the post-transcriptional modifications

昆虫胚胎后发育代谢调控机制研究，重点关注转录后修饰

• 批准号: 62304009
• 负责人: OHNISHI Eiji
• 金额: $ 5.12万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62304009/
• 关键词: Insects; Post-embryonic Development; Metabolic Control; 転字後修飾; 後記発生; 転写後修飾

In post-embryonic development of insects, drastic changes in metabolism could be found. Mechanisms of phenoloxidase activation have been analyzed in relation to cuticulogenesis and to defense mechanism. Ashida analyzed cascade system, involving several proteases, of pro-phenoloxidase of bombyx hemoymph. Yamazaki studied laccase-type oxidase in newly ecdysed cuticles. Ohnishi analyzed structure of the fragment released the prophenoloxidase upon activation. Natori has worked on defense system of the fleshfly, Sarcophage, and analyzed control mechanism of biosynthesis of 2 kinds of peptides, which are inducible by injury. Using affinity chromatography of chitin, Tomino isolated several cuticle-specific proteins and studied temporal pattern of expression of these proteins. Chino studied lipophorin system of locust as modified by adipokinetic hormone. Takahashi isolated cGMP-dependent protein kinase from Bombyx. Yamashita studied egg-specific protein and its protease of Bombyx eggs. Kageyama isolated new type of thiolprotease from Bombyx eggs. These studies revealed various phases of metabolic control mechanisms of insects and the mechanisms were analyzed at a molecular level.

在昆虫的胚胎后发育中，可以发现新陈代谢的剧烈变化。已经分析了与角质生成和防御机制相关的酚氧化酶激活机制。 Ashida 分析了家蚕血淋巴前酚氧化酶的级联系统，涉及几种蛋白酶。山崎研究了新蜕皮角质层中的漆酶型氧化酶。 Ohnishi 分析了激活后释放酚氧化酶原的片段的结构。 Natori研究了肉蝇的防御系统Sarcophage，并分析了损伤诱导的2种肽生物合成的控制机制。 Tomino 使用几丁质亲和层析分离了几种角质层特异性蛋白质，并研究了这些蛋白质表达的时间模式。 Chino研究了脂肪运动激素修饰的蝗虫脂蛋白系统。 Takahashi 从 Bombyx 中分离出 cGMP 依赖性蛋白激酶。山下研究了家蚕卵的卵特异性蛋白质及其蛋白酶。 Kageyama 从家蚕卵中分离出新型硫醇蛋白酶。这些研究揭示了昆虫代谢控制机制的各个阶段，并在分子水平上分析了这些机制。

项目成果

Ando.K.;Natori,S.: Biochemistry. 27. 1715-1722 (1988)

Ando.K.；Natori,S.：生物化学。

Izumi,S.,;Yamasaki,K.,;Tomino,S.;Chnio,H.: "Biosynthesis of apolipophorin III by the fatbody in locust" J.Lipid Research. 28. 667-672 (1987)

Izumi,S.,;Yamasaki,K.,;Tomino,S.;Chnio,H.：“蝗虫脂肪体生物合成载脂蛋白 III”J.Lipid Research。

Hiramoto,N.,;Fujimoto,Y.;Kakinuma,K.,;Ikekawa,N.;Ohnishi, E.: "Ecdysteroid conjugates in the ovaries of the silkworm, Bomb s mori: 3-Phosphates of 2,22-dideoxy-20-hydroxyecdysone and of bombycosterol." Experientia. 44. 623-625 (1988)

Hiramoto,N.,;Fujimoto,Y.;Kakinuma,K.,;Ikekawa,N.;Ohnishi,E.：“蚕卵巢中的蜕皮类固醇结合物，Bomb s mori：2,22-二脱氧的 3-磷酸盐

Sakurai,H.;Fujii,T.;Izumi,S.;Tomino,S.: J.Biological Chemistry. 262. 7876-7880 (1988)

樱井，H.；藤井，T.；泉，S.；富野，S.：J.生物化学。

Indrasith,L.I.;Sasaki,T.;Yamashita,O.: 263. 1988. (1045ー1051)

Indrasith，L.I.; 山下，O.：263。1988。（1045-1051）