Studies on the mechanisms of metabolic control in post-embryonic development of insects, emphasizing on the post-transcriptional modifications

在昆虫的胚胎后期发育中，可以发现代谢的剧烈变化。对酚氧化酶的活化机制从表皮形成和防御机制两方面进行了分析。Ashida分析了家蚕血淋巴前酚氧化酶的级联系统，涉及几种蛋白酶。Yamazaki研究了新蜕皮角质层中的漆酶型氧化酶。Ohnishi分析了片段在激活后释放的酚氧化酶的结构。Natori对肉蝇的防御系统——石质噬菌体进行了研究，分析了2种损伤诱导多肽的生物合成控制机制。利用几丁质的亲和层析，Tomino分离了几个表皮特异性蛋白，并研究了这些蛋白的时间表达模式。中国对蝗虫脂磷脂系统进行了脂动激素修饰的研究。Takahashi从家蚕中分离到了cgmp依赖性蛋白激酶。Yamashita研究了家蚕卵的卵特异性蛋白及其蛋白酶。Kageyama从家蚕卵中分离到一种新型巯基蛋白酶。这些研究揭示了昆虫代谢控制的不同阶段机制，并在分子水平上分析了机制。

In post-embryonic development of insects, drastic changes in metabolism could be found. Mechanisms of phenoloxidase activation have been analyzed in relation to cuticulogenesis and to defense mechanism. Ashida analyzed cascade system, involving several proteases, of pro-phenoloxidase of bombyx hemoymph. Yamazaki studied laccase-type oxidase in newly ecdysed cuticles. Ohnishi analyzed structure of the fragment released the prophenoloxidase upon activation. Natori has worked on defense system of the fleshfly, Sarcophage, and analyzed control mechanism of biosynthesis of 2 kinds of peptides, which are inducible by injury. Using affinity chromatography of chitin, Tomino isolated several cuticle-specific proteins and studied temporal pattern of expression of these proteins. Chino studied lipophorin system of locust as modified by adipokinetic hormone. Takahashi isolated cGMP-dependent protein kinase from Bombyx. Yamashita studied egg-specific protein and its protease of Bombyx eggs. Kageyama isolated new type of thiolprotease from Bombyx eggs. These studies revealed various phases of metabolic control mechanisms of insects and the mechanisms were analyzed at a molecular level.