Forensic Applications of ELISA

ELISA 的法医应用

基本信息

批准号：
62304039
负责人：
TAMAKI Yoshihiro
金额：
$ 1.6万
依托单位：
Medical College of Oita
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62304039/
关键词：
ELISA Personal identification Identification of human body fluids and stains Differntial ABO grouping of semen in mixed stains GM typing 混合体液のABO型判定 GM型判定パラコートの微量定量エライザ血痕検査体液斑検査血液型検査種属鑑別 Gm型判定体液のABO型判定人尿斑の同定ドットELIS

项目摘要

1. Monoclonal antibodies (MoAbs) to ABO and MN antigens, glycophorin A and B, IgG allotypes G3M G and T, A2-seminoglycoprotein (A2SGP), and paraquat were produced for use in ELISAs.2. Human blood could be identified by inhibition-ELISAs using ALB, IgG, A2M, HP, TF, GC, A2HS, BF, and HF as indicator proteins. Human urinary stains were best identified by a capture ELISA using anti-uromucoid as the capture antibody.3. Automation and data processing for ABO grouping of body fluids were effected by assembly of a reaction unit containing a plate coated with stain-derived, detergent-solubilized antigen, and an automatic reading unit.4. A simple dot-immunobinding (dot-ELISA) method for GM typing was developed using MoAbs to G3M G and G3M T. The method is well suited for routine forensic casework.5. A competitive ELISA for paraquat was developed which enabled determination of 1-10 ng/ml of the pesticide.6. Saliva, semen, and blood in mixed stains could be differentially ABO grouped by capture ELISAs using corresponding antibodies. In particular, home-made MoAbs to AB-carrying A2SGR specifically captured the corresponding protein, thereby enabling correct ABO grouping of semen mixed with vaginal fluids. Thus the method appears useful in rape casework.7. Production of MoAbs to IgG allotypes GlM F and GlM X is in progress, since four GM monoclonals are required for routine GM typing of Japanese.

1。抗ABO和MN抗原，糖蛋白A和B，IgG同型G3M G和T，A2-脱糖糖蛋白（A2SGP）和Paraquat的单克隆抗体（MOABS）和paraquat用于Elisas.2。可以使用ALB，IgG，A2M，HP，TF，GC，A2HS，BF和HF作为指示蛋白来鉴定人血液。最好通过使用抗尿素类动物作为捕获抗体的捕获ELISA来最好地识别人尿染色。3。通过组装装有涂有染色的，洗涤剂 - 溶解的抗原和自动读数单元的反应单元组装的反应单元和自动读取单元，对体液进行ABO分组的自动化和数据处理。4。使用MoAbs到G3M G和G3M T开发了一种简单的DOT-DOT-IMMUNOBINDING（DOT-ELISA）方法。该方法非常适合常规法医案例工作。5。开发了一种竞争性ELISA，可确定1-10 ng/ml的农药。6。混合污渍中的唾液，精液和血液可以通过使用相应的抗体捕获ELISA分组。特别是，自制的摩押架以携带A2SGR的A2SGR专门捕获了相应的蛋白质，从而使精液与阴道液混合了正确的ABO分组。因此，该方法在强奸案件中似乎有用7。由于日语常规GM键入所需的四个GM单克隆，因此正在进行MoAbs为IgG Alotypes GLM F和GLM X的生产。