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Forensic Applications of ELISA

ELISA 的法医应用

基本信息

批准号：
62304039
负责人：
TAMAKI Yoshihiro
金额：
$ 1.6万
依托单位：
Medical College of Oita
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62304039/
关键词：
ELISA Personal identification Identification of human body fluids and stains Differntial ABO grouping of semen in mixed stains GM typing 混合体液のABO型判定 GM型判定パラコートの微量定量エライザ血痕検査体液斑検査血液型検査種属鑑別 Gm型判定体液のABO型判定人尿斑の同定ドットELIS

项目摘要

1. Monoclonal antibodies (MoAbs) to ABO and MN antigens, glycophorin A and B, IgG allotypes G3M G and T, A2-seminoglycoprotein (A2SGP), and paraquat were produced for use in ELISAs.2. Human blood could be identified by inhibition-ELISAs using ALB, IgG, A2M, HP, TF, GC, A2HS, BF, and HF as indicator proteins. Human urinary stains were best identified by a capture ELISA using anti-uromucoid as the capture antibody.3. Automation and data processing for ABO grouping of body fluids were effected by assembly of a reaction unit containing a plate coated with stain-derived, detergent-solubilized antigen, and an automatic reading unit.4. A simple dot-immunobinding (dot-ELISA) method for GM typing was developed using MoAbs to G3M G and G3M T. The method is well suited for routine forensic casework.5. A competitive ELISA for paraquat was developed which enabled determination of 1-10 ng/ml of the pesticide.6. Saliva, semen, and blood in mixed stains could be differentially ABO grouped by capture ELISAs using corresponding antibodies. In particular, home-made MoAbs to AB-carrying A2SGR specifically captured the corresponding protein, thereby enabling correct ABO grouping of semen mixed with vaginal fluids. Thus the method appears useful in rape casework.7. Production of MoAbs to IgG allotypes GlM F and GlM X is in progress, since four GM monoclonals are required for routine GM typing of Japanese.

1.制备ABO和MN抗原、血型糖蛋白A和B、IgG同种异型G 3 M G和T、A2-β糖蛋白（A2 SGP）和百草枯的单克隆抗体（MoAb），用于ELISA。采用ALB、IgG、A2 M、HP、TF、GC、A2 HS、BF和HF作为指示蛋白，可通过抑制ELISA鉴定人血液。人尿染色最好通过使用抗尿粘蛋白作为捕获抗体的捕获ELISA来鉴定.通过组装反应单元和自动阅读单元实现体液ABO分型的自动化和数据处理，所述反应单元包含涂有染色衍生的、洗涤剂溶解的抗原的板。用抗G3 MG和G3 MT单克隆抗体建立了一种简便的斑点免疫结合（dot-ELISA）GM分型方法。该方法非常适合于日常法医案件工作。开发了百草枯的竞争性ELISA，能够测定1-10 ng/ml的农药。混合斑中的唾液、精液和血液可通过使用相应抗体的捕获ELISA进行区分ABO分组。特别是，针对携带AB的A2 SGR的自制MoAb特异性地捕获了相应的蛋白质，从而能够对与阴道液混合的精液进行正确的ABO定型。因此，该方法在强奸案件中是有用的。IgG同种异型GlM F和GlM X的单克隆抗体的生产正在进行中，因为日本人的常规GM分型需要四种GM单克隆抗体。