Investigation of plant tumor using various transformation systems

使用各种转化系统研究植物肿瘤

• 批准号: 62540518
• 负责人: HASEZAWA Seiichiro
• 金额: $ 1.28万
• 依托单位: Seijo Junior College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62540518/
• 关键词: transformation; tumor; crown gall; Agrobacterium; T-DNA; spheroplast; プロトプラスト; 感染過程; TーDNA; スフェロプラストラプロトプラスト

The projects which we had prearranged were as follows. 1) analysis of tobacco transformants obtained by the spheroplast method. 2) analysis of tobacco transformants obtained by the co-culture method. 3) observation of interaction between Agrobacterium and plant cells on crown gall formation.With regard to 1) and 2) item, the transformants were classified in some morphological groups. These transformants in the same morphological group were similar in growth rate, hardness, opine and hormones. The T-DNA structures of them were also examined (T.Hoshino, S.Hasezawa, K.Syono).With regard to 3) item, the attachment of Agrobacterium to the surface of plant cells were observed by a scanning electron microscopy (N.Terouchi, S.Hasezawa, ---, K.Syono).The analysis of rice transformants were also carried out. The projects as described above have been almost accomplished.

我们预先安排的项目如下。1)球质体法获得的烟草变形分析。2)共培养法获得的烟草转化体分析。3)农杆菌与植物细胞相互作用对植物冠瘿形成的影响。在1)和2)项上，转化子被划分为若干形态类群。同种形态组的转化体在生长速度、硬度、激素和胸腺激素等指标上基本一致。对它们的T-DNA结构也进行了检测（T.Hoshino， S.Hasezawa， K.Syono）。关于3)项，用扫描电镜观察了农杆菌在植物细胞表面的附着（N.Terouchi， S.Hasezawa，——，K.Syono）。对水稻的转化体进行了分析。上述项目已基本完成。

项目成果

Seiichiro Hasezawa;Akiko Baba;Kunihiko Syono: Biotechnogy in Agriculture and Forestry,BerlinーHeidelbergーNew York.

Seiichiro Hasezawa；Akiko Baba；Kunihiko Syono：农业和林业生物技术，柏林-海德堡-纽约。

馳澤盛一郎: "植物バイオテクノロジー事典" 朝倉書店,

长谷泽诚一郎：《植物生物技术百科全书》朝仓书店，

Seiichiro Hasezawa; Akiko Baba ; Kunihiko Syono: "Protoplast culture and transformation studies on rice." ibid.

长泽诚一郎；

Nobuyuki Terouchi ; Seiichiro Hasezawa ; Kunihiko Syono: "Scanning electron microscopic studies on the attachment of Agrobacterium tumefacieus to the surface of plant cells"

寺内伸之；

馳澤盛一郎,長田敏行: 植物生産システム実用事典.

Seiichiro Hasezawa，Toshiyuki Nagata：植物生产系统实用百科全书。