The study of plant cell morphogenesis by microtubules

微管对植物细胞形态发生的研究

基本信息

批准号：
10640648
负责人：
HASEZAWA Seiichiro
金额：
$ 2.11万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10640648/
关键词：
micro tubule micro filament cellulose microfibril cell cycle diurnal cycle tobacco BY-2 cells Arabidopsis GFP 表層微小管微小管形成中心 EF-1α BY-2細胞孔辺細胞

项目摘要

The main fruits of this study are as follows. 1) The cortical microtubules (MTs) of guard cells of Vicia fava L. were found to be organized in a radial array at dawn and be destroyed from evening to midnight, suggesting that the radially-organized cortical MTs of them may control diurnal stomatal movement (Fukuda et al. 1998). 2) The involvement of microfilaments (MFs) in determining the orientation and organization of cortical MTs was studied and found that the MFs may play a key role in this process (Hasezawa et al. 1998). 3) The MTs could be organized in aster-like form when partially purified proteins prepared from BY-2 cells were incubated with purified porcine brain tubulins. The role of these structures as MTOC sites and the EF-1α were studied (Kumagai et al. 1999). 4) The cellulose microfibril (CMF) bundles appeared to be deposited on the cortical MTs in taxol-treated BY-2 protoplasts, suggesting that MTs may attach directly to the cellulose-synthesizing complexes, by some form of linkage, and regulate the movement of these complexes in higher plant cells (Hasezawa and Nozaki 1999). 5) The MT changes in mitotic suspension cells of stable transgenic Arabjdopsis, expressing a GFP-TUA6 fusion protein, could be monitored by time-sequence observation. We have succeeded, in the first time, in following the MT dynamics in living plant cells throughout mitosis (Hasezawa et al. 2000).

这项研究的主要成果如下。 1）发现Vicia Fava L.的护罩细胞的皮质微管（MTS）在黎明时在径向阵列中组织起来，并从什至到午夜，这表明它们的径向组织的MTS可能会控制昼夜气孔运动（Fukuda等人1998年）。 2）微丝（MFS）参与确定皮质MTS的取向和组织的研究，发现MFS可能在此过程中起关键作用（Hasezawa等，1998）。 3）当将从By-2细胞制备的部分纯化的蛋白与纯化的猪脑微管孵育时，MT可以以星状形式组织。这些结构作为MTOC位点和EF-1α的作用是研究的（Kumagai等，1999）。 4）纤维素微纤维（CMF）捆绑包似乎沉积在紫杉醇治疗的2个原生质体中的皮质MT上，这表明MT可以通过某种形式的链接形式直接附着在纤维素合成的复合物上，并通过某种形式的链接，并调节这些复合物在较高的植物细胞中的运动（Haszawa和NoNONONON NON NON NOSEZAWA和NONON NONECHEZAWA和NOTON）。 5）可以通过时间序列观察来监测稳定的转基因阿拉伯jdopsis（表达GFP-TUA6融合蛋白）的有丝分裂悬浮液细胞的MT变化。在整个有丝分裂过程中，我们首次成功遵循活植物细胞的MT动态（Hasezawa等，2000）。