DETERMINATION OF HEAVY METALS USING ISFET TYPE UREA SENSOR.

对酶ISFET传感器对重金属离子的电位响应进行了评价。它是利用在这项研究中，这是基于对重金属离子的抑制脲酶的敏感性。我们已经证明，作为一个有效的和简单的电位传感器检测铜，汞，铅和镉离子。利用ISFET和固定化脲酶膜构建了酶-ISFET传感器。测定过程包括脲酶与样品中的重金属离子的反应，然后酶反应和膜结合脲酶活性的电化学测定。分析结果直接由传感器的输出电压显示，在0-10 mg/l范围内，获得了初始响应速率与重金属离子浓度的关系。用该膜制作的ISFET的响应可保持两周，且制作新膜也很容易。

A critical evaluation of the potentiometric response of enzyme ISFET sensor to heavy metal ions is described. It is utilized in this study, which is based on the susceptibility of urease to inhibition by heavy metal ions. We have demonstrated that as an effective and simple potentiometric sensor for detecting copper, mercury, lead and cadmium ions. The enzyme-ISFET sensor was constructed using as immobilized urease membrane and a ISFET. The assay procedure involves the reaction of urease and heavy metal ions in samples and after that the enzyme reaction and the electrochemical determination of membrane bound urease activity. The analytical result is directly displayed by the output voltage of the sensor.In this study, a relationship was obtained between the initial rate of response and the heavy metal ion concentrations in a range of 0-10 mg/l. The response of ISFET with the membrane was kept for two weeks and making a new membrane was also easy.