DETERMINATION OF HEAVY METALS USING ISFET TYPE UREA SENSOR.

描述了酶ISFET传感器对重金属离子的电位响应的关键评估。基于脲酶对重金属离子抑制的敏感性，本研究利用了它。我们已经证明，作为一个有效的和简单的电位传感器检测铜，汞，铅和镉离子。采用固定化脲酶膜和ISFET构建酶-ISFET传感器。分析过程包括样品中脲酶与重金属离子的反应，然后进行酶反应和电化学测定膜结合脲酶活性。传感器的输出电压直接显示分析结果。在0 ~ 10 mg/l范围内，得到了初始响应速率与重金属离子浓度之间的关系。ISFET与膜的反应保持了两周，制备新膜也很容易。

A critical evaluation of the potentiometric response of enzyme ISFET sensor to heavy metal ions is described. It is utilized in this study, which is based on the susceptibility of urease to inhibition by heavy metal ions. We have demonstrated that as an effective and simple potentiometric sensor for detecting copper, mercury, lead and cadmium ions. The enzyme-ISFET sensor was constructed using as immobilized urease membrane and a ISFET. The assay procedure involves the reaction of urease and heavy metal ions in samples and after that the enzyme reaction and the electrochemical determination of membrane bound urease activity. The analytical result is directly displayed by the output voltage of the sensor.In this study, a relationship was obtained between the initial rate of response and the heavy metal ion concentrations in a range of 0-10 mg/l. The response of ISFET with the membrane was kept for two weeks and making a new membrane was also easy.