Nucleic acids analysis and recombination among strains of cucumber mosaic virus.

本研究的目的是通过双链RNA （DS-RNA）分析比较几种黄瓜花叶病毒（CMV）分离株。从感染巨细胞病毒的叶片中提取复制形式-ds- rna (RF-ds-RNA)，并进行银染色电泳。根据RF-ds-RNA的电泳迁移模式可以区分CMV分离株。2. 除了基因组RNA1-4外，一些CMV分离株还具有影响症状的卫星RNA （CARNA5）。用非放射性光生物素标记CARNA5的Ds-RNA，成功地用作核酸杂交探针。3. 菊花轻度斑驳病毒（Chrysanthemum mild mottle virus， CMMV）是新近从辣椒和矮牵牛中分离到的cmv病毒群中的一员。将调节CMMV症状的CMMV卫星样RNA5与CARNA5杂交。4. 巨细胞病毒的RF-ds-RNA可电穿孔到烟草叶肉原生质体，但不具有传染性。

The purpose of this study was to compare several isolates of cucumber mosaic virus (CMV) by double-stranded RNA (DS-RNA) analysis.1. Replicative form-ds-RNA (RF-ds-RNA) was extracted from leaves infected with CMV and electrophoresed with silver-staining. CMV isolates can be distinguished on the basis of electrophoretic migration pattern of RF-ds-RNA. 2. In addition to genomic RNA1-4, some isolates of CMV have a satellite RNA (CARNA5) which influences symtoms. Ds-RNA of CARNA5 was labeled with non-radioactive photobiotin and was found to be successfully used as probe of nucleic acid hybridization. 3. Chrysanthemum mild mottle virus (CMMV), a member of CMV-group, was newly isolated from pepper and petunia. Satellite-like RNA5 of CMMV which modulates the symptoms of CMMV was hybridized with CARNA5. 4. RF-ds-RNA of CMV was electro-porated to tobacco mesophyll protoplast but was not infectious.