Anarysis of vector transmission, genome straucture
媒介传播分析、基因组结构
基本信息
- 批准号:14560036
- 负责人:
- 金额:$ 2.18万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Molecular cloning and sequencing of plant virus genomes has been carried out during the past decade. One objective of molecular analysis is improvement of the methods to detect and diagnose pathogenic viruses. For molecular analysis, viral nucleic acids are usually extracted from purified virus preparations. However, there are many recalcitrant viruses that are unable to purify by current methods and, therefore, the viral nucleic acid samples and their antisera are not available. Cucumber yellows virus(CuYV) is one of such recalcitrant viruses. In this research, the application of dsRNA extraction technique from plants was applied to get viral RNA, replicative form of dsRNA(RF-dsRNA). The first objective of this research was the production of cDNA clones of CuYV and complete nucleotide sequence of CuYV genome was determined. This is the first record in the world and suggests that CuYV is a member of the genus Crinivirus. Occurrence of CuYV was also detected in Indonesia but not in Pakistan. However, the virus could not be detected from its whitefly vector by PCR. In this research Tomato infectious chlorosis virus, a member of criniviruses, was newly recognized in Japan. We have also attempted that the dsRNA extraction technique was used to construct a plasmid to express the viral coat protein in E.coli. Finally production of the antiserum against carrot cryptic virus was succeeded, indicating that this method is useful to prepare antisera against ciriniviruses for which no antisera are available in present.
植物病毒基因组的分子克隆和测序在过去的十年中已经完成。分子分析的目的之一是改进检测和诊断致病病毒的方法。对于分子分析,通常从纯化的病毒制剂中提取病毒核酸。然而,存在许多不能通过当前方法纯化的寄生虫病毒,因此,病毒核酸样品和它们的抗血清不可用。黄瓜黄化病毒(Cucumber Yellows Virus,CuYV)就是其中的一种。本研究应用植物dsRNA提取技术,从植物中提取病毒RNA,复制型dsRNA(RF-dsRNA)。本研究的第一个目的是制备CuYV的cDNA克隆,并测定CuYV基因组的完整核苷酸序列。这是世界上第一个记录,表明CuYV是Crinivirus属的成员。在印度尼西亚也发现了CuYV,但在巴基斯坦没有发现。然而,该病毒不能通过PCR从其粉虱载体中检测到。番茄传染性褪绿病毒是日本新近发现的一种蔓状病毒属病毒。我们还尝试使用dsRNA提取技术来构建质粒以在大肠杆菌中表达病毒外壳蛋白。最后成功地制备了抗胡萝卜潜病毒的抗血清,表明该方法可用于制备目前尚无抗胡萝卜潜病毒血清的抗血清。
项目成果
期刊论文数量(16)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hartono, S., Natsuaki, T., Genda, Y., Okuda, S.: "Nucleotide sequence and genome organization of yellows virus, a member of the genus Crinivirus"Journal of General Virology. 84・4. 1007-1012 (2003)
Hartono, S.、Natsuaki, T.、Genda, Y.、Okuda, S.:“病毒属黄病毒的核苷酸序列和基因组结构”普通病毒学杂志 84・4。 2003)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
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A closterovirus detected from cucumber showing yellowing symptoms in Indonesia.
在印度尼西亚,从出现泛黄症状的黄瓜中检测到一种线形病毒。
- DOI:
- 发表时间:2002
- 期刊:
- 影响因子:0
- 作者:Hartono;S.;Natsuaki;T.;Okuda;S.
- 通讯作者:S.
Hartono, S., Natuaki, T., Genda, Y., Okuda, S.: "Nucleotide sequence and genome organization of cucumber yellows virus, a member of the genus Crinivirus"Journal of General Virology. 84・4. 1007-1012 (2003)
Hartono, S.、Natuaki, T.、Genda, Y.、Okuda, S.:“克里尼病毒属成员黄瓜黄化病毒的核苷酸序列和基因组结构”普通病毒学杂志 84・4。 (2003)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Yellowing disease of tomatoes caused by Tomato infectious chlorosis virus newly recognized in Japan
- DOI:10.1007/s10327-002-0015-x
- 发表时间:2003-02
- 期刊:
- 影响因子:1.2
- 作者:S. Hartono;T. Natsuaki;H. Sayama;H. Atarashi;S. Okuda
- 通讯作者:S. Hartono;T. Natsuaki;H. Sayama;H. Atarashi;S. Okuda
Hartono, S., Natsuaki, T., Sayama, H., Atarashi, H., Okuda, S.: "Yellowing disease of tomatoes caused by Tomato infectious chlorosis virus newly recognized in Japan"Journal of General Plant Pathology. 69・1. 61-64 (2003)
Hartono,S.,Natsuaki,T.,Sayama,H.,Atarashi,H.,Okuda,S.:“日本新发现的番茄传染性褪绿病毒引起的番茄黄化病”普通植物病理学杂志69・1。 .61-64 (2003)
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- 影响因子:0
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OKUDA Seiichi其他文献
OKUDA Seiichi的其他文献
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{{ truncateString('OKUDA Seiichi', 18)}}的其他基金
Research on integrated control methods of whitefly-transmitted viruses in Indonesia and its application to Japan
印度尼西亚烟粉虱传播病毒综合防治方法研究及其在日本的应用
- 批准号:
20405024 - 财政年份:2008
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Studies on Sustainable Agriculture and on Genetic Resources
可持续农业和遗传资源研究
- 批准号:
11695069 - 财政年份:1999
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Template-switching of CMV-RNA at replication.
CMV-RNA 在复制时的模板转换。
- 批准号:
10660044 - 财政年份:1998
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Nucleic acids analysis and recombination among strains of cucumber mosaic virus.
黄瓜花叶病毒株间的核酸分析和重组。
- 批准号:
62560036 - 财政年份:1987
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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