CHOLINE METABOLISM AND AMOUNTS OF C_1-COMPOUNDS IN RAT LIVER

在胆碱缺乏的大鼠中，研究了以氨基酸混合物为主的饲料中添加和不添加吡咯喹啉醌(PQQ)时，大鼠小肠中肝胆碱脱氢酶(EC 1.1.99.1)和二胺氧化酶(EC 1.4.3.6)的活性变化。无论有无PQQ，常规饲料中的胆碱缺乏均可引起严重的脂肪肝。然而，无论饲喂PQQ与否，肝脏中胆碱脱氢酶和小肠中二胺氧化酶的活性没有显著变化。当无菌大鼠喂食以氨基酸混合物为主并含有足够胆碱的食物时，也得到了类似的结果。

In the choline deficient rats, the activities of hepatic choline dehydrogenase (EC 1.1.99.1) and diamine oxidase (EC 1.4.3.6)from small intestine, both are assumed to contain a new type coenzyme, covalently-bound pyrroloquinoline quinone (PQQ), were investigated when rats were fed diets consisting mainly of amino acid mixtures with of without PQQ.Choline deficiency from diet induced severe fatty liver when conventional rats were fed a diet consisting of amino acid mixtures in spite of the presence or absence of PQQ. However, the activities of choline dehydrogenase in liver together with diamine oxidase in small intestine did not alter significantly, whether the diet was fed with or without PQQ. Similar results were obtained when the germ-free rats were fed diets consisting mainly of amino acid mixtures with enough choline.