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HPLC Analysis of Vitamin B_6 Derivatives

维生素 B_6 衍生物的 HPLC 分析

基本信息

批准号：
05660133
负责人：
TSUGE Haruhito
金额：
$ 1.34万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660133/
关键词：
Vitamin B_6 HPLC Analysis Vitamin B_6 contents in food Microbioassay マイクロバイオアッセイ PLP ピリドキサミン5′-リン酸 MPLC分析血中ビタミンB_6濃度

项目摘要

In order to determine the contents of vitamin B_6 derivatives, which are ubiquitously found in natural products, isocratic reversed-phase HPLC method, newly developed in our laboratory, was examined whether or not the method was applicable to the analysis from 1) blood, urine or some mammalian organs and 2) complex food samples. The determined data offer the standard for the recommended dietary allowance (RDA) for vitamin B_6. For the project 1) , our HPLC method gave a powerful means to analyze the concentration changes of vitamin B_6 derivatives in some organs as well as in blood, urine. Especially, pyridoxal 5'-phosphate (PLP) , a main component of vitamin B_6 derivatives, was effectively analyzed after the conversion of it to pyridoxic acid 5'-phosphate (PIC-P) by means of KCN treatment.On the other hand, the analysis of total vitamin B_6 contents in some foods and food materials when analyzed after perchloric acid extraction, was estimated just lower than the values which were obtained by a microbiological assay using Saccharomyces cerevisiae ATCC 9080 as well as by data from HPLC method after HCl-hydrolysis.In summary, the extraction procedure of vitamin B_6 derivatives from complex food matrices have to be reexamined precisely to make the method applicable to the food analyzes.

为了测定天然产物中普遍存在的维生素B_6衍生物的含量，我们实验室新开发了等度反相HPLC方法，检验了该方法是否适用于1）血液、尿液或一些哺乳动物器官和2）复杂食品样品的分析。确定的数据提供了维生素 B_6 的推荐膳食摄入量 (RDA) 标准。对于项目1)，我们的HPLC方法为分析某些器官以及血液、尿液中维生素B_6衍生物的浓度变化提供了有力的手段。特别是，维生素B_6衍生物的主要成分吡哆醛5'-磷酸(PLP)，通过KCN处理转化为吡哆酸5'-磷酸(PIC-P)后，可以得到有效的分析。另一方面，一些食品和食品原料中经高氯酸提取后分析的维生素B_6总含量，估计略低于预测值。通过使用酿酒酵母 ATCC 9080 进行微生物测定以及 HCl 水解后的 HPLC 方法获得的数据。总之，必须重新精确地重新检查从复杂食品基质中提取维生素 B_6 衍生物的程序，以使该方法适用于食品分析。