Biochemical Study on Terminal Oxidase of Bacterial Respiratory Chain

细胞色素o氧化酶是一种广泛存在于多种细菌中的酶，本文通过比较埃希氏菌、葡萄杆菌、醋酸杆菌和假单胞菌的结构和功能，对其进行了研究。并将该氧化酶与另一末端氧化酶的功能进行了比较。1)大肠杆菌末端氧化酶：呼吸链中含有细胞色素o和d作为末端氧化酶，其合成受培养基中氧浓度的调节。细胞色素o氧化酶能够与泛醇反应，并在重组的蛋白脂质体中产生膜电位。此外，氧化酶还能与蛋白脂质体中的Q-8和葡萄糖脱氢酶（GDH）重建葡萄糖氧化酶呼吸链。2) G. subboxydans末端氧化酶：呼吸链分支有细胞色素o和由细胞色素c组成的不产生能量的旁路，旁路仅受培养基pH的影响。细胞色素o与大肠杆菌具有几乎相同的结构和功能，因此能够与泛醇反应并产生能量。然而，该氧化酶与大肠杆菌的细胞色素o在免疫上有一定的差异，而且，该氧化酶还能与Q-10和gdh重建葡萄糖氧化酶呼吸链。3)乙酰a.b乙酰的末端氧化酶：呼吸链中含有细胞色素o和细胞色素a作为末端氧化酶，其末端氧化酶随培养条件、摇动或静止而变化。细胞色素o具有与上述酶几乎相同的结构和功能。此外，细胞色素a的结构和功能与细胞色素o相似。目前，细胞色素o与细胞色素a之间的关系尚不清楚。4)铜绿假单胞菌末端氧化酶：呼吸链以细胞色素o为末端氧化酶，并有不敏感的氰化物旁路。细胞色素o与其他细胞色素o有很大的不同，它与色素c反应，但不与泛醇反应，但在产生能量的性质上没有区别。少

Cytochrome o oxidase, which functions extensively in a wide variety of bacteria, was investigated by comparing its structure and function among several bacteria such as Escherichia, Gluconobacter, Acetobacter and Pseudomonas. The oxidase was also oxidase was also compared in their function with another terminal oxidase coexistion in the respective strains.1) Terminal oxidase of E. coli: The respiratory chain contains cytochromes o and d as terminal oxidases, the synthesis of which is regulated by oxygen concentration in the medium. The cytochrome o oxidase was able to react with ubiquinol, and to generate a membrane potential in the reconstituted proteoliposomes. Furthermore, the oxidase was able to reconstitute glucose oxidase respiratory chain with Q-8 and glucose dehydrogenase (GDH) in the proteoliposomes.2) Terminal oxidase of G. subboxydans: The respiratory chain branched with cytochrome o and a non-energy-generating bypass consisting of a cytochrome c. The bypass only was changed … More by pH of the medium. The cytochrome o had almost the same structure and function as that of E. coli, and thus was able to react with ubiquinol and to generate an energy. However, the oxidase was somewhat different immunologically from e. coli cytochrome o. Furthermore, the oxidase was also able to reconstitute glucose oxidase respiratory chain with Q-10 and GDH.3) Terminal oxidase of A. aceti: The respiratory chain contained cytochromes o and a as terminal oxidases, which changed depending on culture conditions, shaking or stationary. The cytochrome o had almost the same structure and function as the enzyme described above. Furthermore, the structure and function of the cytochrome a was similar to those of the cytochrome o. At this moment, however, the relationship between the cytochromes o and a is not clear.4) Terminal oxidase of P. aerugiosa: The respiratory chain had cytochrome o as the terminal oxidase, and further a cyanide insensitive bypass. The cytochrome o was extensively different from others, reacting chtochrome c but not with ubiquinol, but not different in the property to generate an energy. Less