Studies on Metabolizing Activities toward Nitrogen- and Sugar-Compounds in Bifidobacteria and Their Application

双歧杆菌氮、糖化合物代谢活性研究及其应用

• 批准号: 62560101
• 负责人: YANO Toshihiro
• 金额: $ 1.15万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62560101/
• 关键词: Bifidobacteria; Enzymatic synthesis of oligosaccharides assimilable by bifidobacteria; Glutamate dehydrogenase; Glutamine synthetase; -D-Fucosylmannitol; -D-Glucosidase I; 特異的資化糖の酵素合成; βーDグルコシダーゼI; アンモニア代謝; 糖質代謝

1. Bifidobacterial enzymes participating in ammonia metabolism.(1) Inactivation of glutamine synthetase (GS) in bifidobacteria was caused by enzymatic adenylylation of GS protein, and the adenylylation enzyme of bifidobacterium pseudolongum was characterized. The changes in the properties of the GS-reactions after the action of the enzyme were different from the findings obtained with enterobacteria but similar to those in a few species of actinomycetes.(2) Glutamate dehydrogenases (GDHs) were purified from some bifidobacteria. Their enzymatic properties indicated that the enzymes catalyze reductive amination pysiologically rather than oxidative deamination, and suggested that they play some important role in ammonia metabolism together with GS.(3) Analyses of variation of ammonia concentration in feces of a breast-fed infant suggested that bifidobacterial cells and GS might be concerned with elimination of ammonia in the lower intestines.2. Properties and utilization of oligosaccharid … More e-hydrolyzing enzymes of bifidobacteria.(1) A simple method was established for prepartion of a new -D-glucosidase ( -D-glucosid-ase I) from bifidobacterium breve 203, which has desirable properties for practical use.(2) -D-glucosidase I hydrolyzed not only p-nitrophenyl (p-NP) -D-glucoside but also p-BP -D-fucoside and, furtheremore, transferred -D-fucosyl moiety to other sugars or sugar derivatives. By use of the enzyme, -D-fucosylglucose was synthesized in the mixture containing glucose as an acceptor with a yield of 52 % based on -D-fucosyl donor (p-NP -D-fucoside). The product consisted of four isomers ( 1-2, 1-3, 1,4 and 1-6), and it suppoeted specifically bifidobacterial growth. -D-fucosylmannitol was synthesized similarly with a yield of 45 %. It contained at least two isomers, however, their chemical structures and assimilability by bifidobacteria were not revealed in this study.Formation of new substance was confirmed in reaction mixture containing maltose, xylose, glycerol or sorbitol as an acceptor. Less

1.参与氨代谢的双歧杆菌酶。(1)双歧杆菌谷氨酰胺合成酶（GS）的失活是由GS蛋白的腺苷酰化引起的，并对假长双歧杆菌的腺苷酰化酶进行了表征。酶作用后GS反应性质的变化与肠杆菌的结果不同，但与一些放线菌的结果相似。(2)谷氨酸脱氢酶（GDH）是从一些双歧杆菌中纯化得到的。这些酶的酶学性质表明，它们在生理上催化还原胺化而不是氧化脱氨，并表明它们与GS一起在氨代谢中发挥重要作用。(3)对母乳喂养婴儿粪便中氨浓度变化的分析表明，双歧杆菌细胞和GS可能与下消化道氨的排出有关.低聚糖的性质及利用 ...更多信息 双歧杆菌的e-水解酶。(1)建立了一种从短双歧杆菌203中分离纯化β-D-葡萄糖苷酶的简便方法。(2)- D-葡萄糖苷酶Ⅰ不仅水解对硝基苯基（p-NP）-D-葡萄糖苷，而且水解p-BP -D-岩藻糖苷，并将-D-岩藻糖基转移到其它糖或糖衍生物上。通过使用该酶，在含有葡萄糖作为受体的混合物中合成-D-岩藻糖基葡萄糖，基于-D-岩藻糖基供体（p-NP -D-岩藻糖苷），产率为52%。该产物由4种异构体（1-2、1-3、1、4和1-6）组成，并特异性地支持细菌生长。类似地合成了β-D-岩藻糖基甘露醇，产率为45%。它至少含有两种异构体，但它们的化学结构和被双歧杆菌同化的能力在本研究中没有揭示。在含有麦芽糖、木糖、甘油或山梨醇作为受体的反应混合物中，新物质的形成得到了证实。少

项目成果

酒井謙二: Agricultural and Biological Chemistry. 52. (1988)

Kenji Sakai：农业和生物化学 52。（1988）

畑中,勝行: Agricultural and Biological Chemistry.

Hatanaka，Katsuyuki：农业和生物化学。

Tachiki, Takashi: "Glutamine Synthetase-Adenylylating Enzyme from Bifidobacterium pseudolongum" Agricultural and Biological Chemistry.

Tachiki，Takashi：“来自假长双歧杆菌的谷氨酰胺合成酶-腺苷酸化酶”农业和生物化学。

Sakai, Kenji: "Synthesis of -D-Fucosylglucose by Using -D-Glucosidase I of Bifidobacterium breve 203 and Its Assimilation by Bifidobacteria" Agricultural and Biological Chemistry. 53. 313-318 (1989)

Sakai，Kenji：“使用短双歧杆菌 203 的 -D-葡萄糖苷酶 I 合成 -D-岩藻糖基葡萄糖及其通过双歧杆菌的同化”农业和生物化学。

Yano, Toshihiro: "Glutamine Formation from Ammonia and Glutamate in Feces by Coupling of Bifidobacterial Cells and Glutamine Synthetase" Agricultural and Biological Chemistry.

Yano，Toshihiro：“通过双歧杆菌细胞和谷氨酰胺合成酶的偶联从粪便中的氨和谷氨酸形成谷氨酰胺”农业和生物化学。