Construction of host・vector system in mold: Function and structure of extranuclear DNA in fungi
霉菌宿主·载体系统的构建:真菌核外DNA的功能和结构
基本信息
- 批准号:62560103
- 负责人:
- 金额:$ 1.22万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1987
- 资助国家:日本
- 起止时间:1987 至 1988
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Fungi is industrially important microbe and various useful enzymes and organic acids are producing by using fungi cells in a large industrial scale. To breed more useful fungi, we intended to construct host-vector systems available in fungi. For the construction of the system, plasmid DNAs are required to clone and amplify the genes in fungi cells. However, we have no plasmid DNAs available in fungi system, and yeast plasmid DNAs have been utilized, although the yeast plasmid DNAs are unstable in fungi cells and frequently integrated into fungi genomes. To overcome this barrier, the use of plasmid DNAs inherent to fungi is thought to be suitable. So, we screened the plasmid DNAs in various fungi cells. despite the voluminous screening experiments, we could not find the plasmid DNAs, except that plasmid DNA-like molecule was found in Mucor jansence. On the other hand, we purified glyoxalase I and methylglyoxal reductases from Aspergillus niger. We are now attempting to construct host-vector systems by using plasmid DNA we found in M. ransence and genes for these enzymes.
真菌是工业上重要的微生物,并且通过使用真菌细胞以大工业规模生产各种有用的酶和有机酸。为了培育出更多有用的真菌,我们打算构建真菌中可用的宿主-载体系统。为了构建该系统,需要质粒DNA来克隆和扩增真菌细胞中的基因。然而,我们没有质粒DNA可用于真菌系统,并且已经利用酵母质粒DNA,尽管酵母质粒DNA在真菌细胞中不稳定并且经常整合到真菌基因组中。为了克服这一障碍,使用真菌固有的质粒DNA被认为是合适的。因此,我们在各种真菌细胞中筛选质粒DNA。尽管进行了大量的筛选实验,但除了在毛霉中发现了质粒DNA样分子外,我们没有发现质粒DNA。另一方面,我们从尼日尔曲霉中纯化了glycoprotein酶I和甲基乙二醛还原酶。我们现在正试图利用我们在M.这些酶的遗传和基因。
项目成果
期刊论文数量(8)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Yoshiharu Inoue: "Metabolism of 2-oxoaldehydes in mold :Purification and characterization of two methylglyoxal reductases from Aspergillus niger" Eur.J.Biochem.171. 213-218 (1988)
Yoshiharu Inoue:“霉菌中 2-氧醛的代谢:来自黑曲霉的两种甲基乙二醛还原酶的纯化和表征”Eur.J.Biochem.171。
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- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Yoshiharu Inoue.: Eur.J.Biochem.171. 213-218 (1988)
井上义治:Eur.J.Biochem.171。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Yoshiharu Inoue: "Metabolism of 2-oxoaldehydes in mold: Purification and characterization of glyoxalase I from Aspergillus niger." J. Biochem.102. 583-589 (1987)
Yoshiharu Inoue:“霉菌中 2-氧代醛的代谢:黑曲霉乙二醛酶 I 的纯化和表征。”
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MURATA Kousaku其他文献
MURATA Kousaku的其他文献
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{{ truncateString('MURATA Kousaku', 18)}}的其他基金
Infection mechanism of nitrogen-fixing bacteria to nonlegume and its application to construction of agriculture independent on chemical nitrogenous fertilizers
固氮菌对非豆科植物的侵染机制及其在建设非化学氮肥农业中的应用
- 批准号:
24658076 - 财政年份:2012
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Structure and function of complex of substrate-binding protein and macromolecule-specific ABC transpoter
底物结合蛋白与大分子特异性ABC转座子复合物的结构与功能
- 批准号:
23380049 - 财政年份:2011
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular identification of nitrogen-transporter and analysis of nitrogen-fixing reaction in bacteria
细菌氮转运蛋白的分子鉴定及固氮反应分析
- 批准号:
22658026 - 财政年份:2010
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Structure/function relationship and cell surface localization mechanism of bacterial flagelin
细菌鞭毛蛋白的结构/功能关系及细胞表面定位机制
- 批准号:
20380049 - 财政年份:2008
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular basis for cell surface architecture and evolution of bacterial flagellin by structure and unction analysis of the protein
通过蛋白质的结构和功能分析,了解细胞表面结构和细菌鞭毛蛋白进化的分子基础
- 批准号:
17380053 - 财政年份:2005
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$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Biochemical significance of the non-common amino acid sequences specifying the common functions of enzymes, polysaccharide lyases
指定酶、多糖裂解酶的常见功能的非常见氨基酸序列的生化意义
- 批准号:
14360052 - 财政年份:2002
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Structure/function analysis of abundance and transmission mechanism of genetic information invlolved in the biosynthesis of multi-catalytic site enzyme
多催化位点酶生物合成涉及的遗传信息丰度及传递机制的结构/功能分析
- 批准号:
11460039 - 财政年份:1999
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Function and safety of bacterial alginate lyase for the therapy of biofilm-dependent infection of Pseudomonas aeruginosa
细菌藻酸盐裂解酶治疗铜绿假单胞菌生物膜依赖性感染的功能和安全性
- 批准号:
10556017 - 财政年份:1998
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
X-ray diffraction study and reaction mechanism of bacterial alginate lyase
细菌海藻酸裂解酶的X射线衍射研究及反应机制
- 批准号:
07660111 - 财政年份:1995
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Safety assessment of genetically engineered yeast and breeding of non-toxigenic industrially used yeasts
基因工程酵母的安全性评价及无毒工业用酵母的选育
- 批准号:
07556091 - 财政年份:1995
- 资助金额:
$ 1.22万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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